A Novel Line Immunoassay Based on Recombinant Virulence Factors Enables Highly Specific and Sensitive Serologic Diagnosis of Helicobacter pylori Infection
| Autor: | Michael Geppert, Christian Bolz, Michael Vieth, Markus Gerhard, Arne Schneider, Gereon Göttner, Kurt Ulm, Laura Romberg, Erwin Soutschek, Wolfgang Schepp, Petra Wolf, Dirk Walter, Luca Formichella, Christina Nölting, Dirk H. Busch |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Microbiology (medical) Adolescent Virulence Factors Clinical Biochemistry Immunology Population Gene Expression Virulence Sensitivity and Specificity Helicobacter Infections Serology Young Adult Antigen Diagnostic Laboratory Immunology Escherichia coli medicine Humans Immunology and Allergy CagA education Aged Aged 80 and over Immunoassay Antigens Bacterial education.field_of_study Helicobacter pylori biology medicine.diagnostic_test Diagnostic Tests Routine Middle Aged biology.organism_classification Antibodies Bacterial Recombinant Proteins ddc biology.protein Female Antibody |
| Zdroj: | Clinical and Vaccine Immunology. 20:1703-1710 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | Helicobacter pylori colonizes half of the world's population, and infection can lead to ulcers, gastric cancer, and mucosa-associated lymphoid tissue (MALT) lymphoma. Serology is the only test applicable for large-scale, population-based screening, but current tests are hampered by a lack of sensitivity and/or specificity. Also, no serologic test allows the differentiation of type I and type II strains, which is important for predicting the clinical outcome. H. pylori virulence factors have been associated with disease, but direct assessment of virulence factors requires invasive methods to obtain gastric biopsy specimens. Our work aimed at the development of a highly sensitive and specific, noninvasive serologic test to detect immune responses to important H. pylori virulence factors. This line immunoassay system ( recom Line) is based on recombinant proteins. For this assay, six highly immunogenic virulence factors (CagA, VacA, GroEL, gGT, HcpC, and UreA) were expressed in Escherichia coli , purified, and immobilized to nitrocellulose membranes to detect serological immune responses in patient's sera. For the validation of the line assay, a cohort of 500 patients was screened, of which 290 (58.0%) were H. pylori negative and 210 (42.0%) were positive by histology. The assay showed sensitivity and specificity of 97.6% and 96.2%, respectively, compared to histology. In direct comparison to lysate blotting and enzyme-linked immunosorbent assay (ELISA), the recom Line assay had increased discriminatory power. For the assessment of individual risk for gastrointestinal disease, the test must be validated in a larger and defined patient cohort. Taking the data together, the recom Line assay provides a valuable tool for the diagnosis of H. pylori infection. |
| Databáze: | OpenAIRE |
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