An electro-active system of immuno-assay (EASI assay) utilising self assembled monolayer modified electrodes
| Autor: | Steven Howell, Robert Porter, Marika Kyröläinen-Reay, Andy Badley, Paul van der Logt |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Analyte
Estrone Molecular Sequence Data Carbazoles Biomedical Engineering Biophysics Enzyme-Linked Immunosorbent Assay Biosensing Techniques Sensitivity and Specificity chemistry.chemical_compound Antibodies Bispecific Monolayer Electrochemistry medicine Animals Amino Acid Sequence Electrodes Immunoglobulin Fragments Immunoassay Chromatography Base Sequence biology medicine.diagnostic_test Carbazole Self-assembled monolayer General Medicine Amperometry Electronics Medical chemistry biology.protein Antibody Camelids New World Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 16:875-885 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/s0956-5663(01)00207-x |
| Popis: | Most immunoassays currently rely on optical methods for signal generation e.g. in ELISA and rapid assay formats. It has become apparent as in the Glucose sensor market that there is a need for simple direct electrical immuno-sensors. We have investigated the novel use of organic conducting monolayers used as a direct electrochemical detection support for an immuno-reaction. It was found that antibodies raised to a carbazole dimer monolayer could increase the charge movement across that monolayer surface. Antibody fragments were taken from a specific anti-carbazole antibody fragment library and combined with an antibody fragment directed to the hormone estrone 3 glucuronide (E3G), the target antigen to form a bispecific antibody fragment. The device utilised these specific antibody fragments and incorporated them on the top plate of a capillary fill format as the immuno-assay components. The immuno-reaction utilised a competition assay. Free E3G analyte in the sample displaced the bispecific antibody fragment from the immuno-surface leaving it free to bind the carbazole monolayer surface. There the binding was detected using amperometric or coulometric methods. By combining all there element it was possible to develop a sensitive immuno-assay that could detect E3G in a reproducible calibrated fashion down to 10 ng/ml. |
| Databáze: | OpenAIRE |
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