Identification of a conserved region of Plasmodium falciparum MSP3 targeted by biologically active antibodies to improve vaccine design

Autor: Michael Theisen, Pierre Druilhe, Juan-Pedro Mejia, Christian Roussilhon, Soe Soe, Subhash Singh, Giampietro Corradin
Rok vydání: 2003
Předmět:
Male
Protozoan Vaccines
Antigenicity
Plasmodium falciparum
Protozoan Proteins
Antibodies
Protozoan
Peptide
Antigens
Protozoan
Epitope
Immunoglobulin G
Microbiology
Immunocompromised Host
Mice
parasitic diseases
medicine
Immunology and Allergy
Animals
Humans
Merozoite surface protein
Malaria
Falciparum
chemistry.chemical_classification
biology
Animals Antibodies
Protozoan/*immunology Antigens
Protozoan/*chemistry/genetics/*immunology Disease Models
Animal Drug Design Epitopes
B-Lymphocyte Humans Immunocompromised Host Malaria
Falciparum/*immunology/parasitology Male Mice Plasmodium falciparum/*immunology Protozoan Proteins/*chemistry/genetics/*immunology Protozoan Vaccines Recombinant Proteins/immunology
T helper cell
biology.organism_classification
Virology
Recombinant Proteins
Disease Models
Animal
Infectious Diseases
medicine.anatomical_structure
chemistry
Drug Design
biology.protein
Epitopes
B-Lymphocyte
Antibody
Zdroj: Journal of Infectious Diseases, vol. 190, no. 5, pp. 1010-8
ISSN: 0022-1899
Popis: Merozoite surface protein 3 (MSP3) is a target of antibody-dependent cellular inhibition (ADCI), a protective mechanism against Plasmodium falciparum malaria. From the C-terminal half of the molecule, 6 overlapping peptides were chosen to characterize human immune responses. Each peptide defined at least 1 non-cross-reactive B cell epitope. Distinct patterns of antibody responses, by level and IgG subclass distribution, were observed in inhabitants of a malaria-endemic area. Antibodies affinity purified toward each peptide differed in their functional capacity to mediate parasite killing in ADCI assays: 3 of 6 overlapping peptides had a major inhibitory effect on parasite growth. This result was confirmed by the passive transfer of anti-MSP3 antibodies in vivo in a P. falciparum mouse model. T helper cell epitopes were identified in each peptide. Antigenicity and functional assays identified a 70-amino acid conserved domain of MSP3 as a target of biologically active antibodies to be included in future vaccine constructs based on MSP3.
Databáze: OpenAIRE
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