Recent actuality about Bacillus cereus and human milk bank: a new sensitive method for microbiological analysis of pasteurized milk

Autor: Virginie Rigourd, Jean-François Magny, Agnès Ferroni, M Nicloux, Alexandre Lapillonne, Xavier Nassif, Jean-Philippe Barnier, Emmanuelle Bille, Pierre Frange, T Hachem
Rok vydání: 2018
Předmět:
Zdroj: European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 37(7)
ISSN: 1435-4373
Popis: Three cases of Bacillus cereus infection or colonization occurred in the same region in France, and milk from the milk bank was suspected as a possible common source of contamination. All Batches delivered to the three cases complied with the requirements of the bacteriological reference method recommended by good practices guidelines. Still, a retrospective analysis with a more sensitive method showed one batch to contain B. cereus, however straincomparison revealed no epidemiological link betweenisolates from patients and those from the milk. Consequently, in accordance with the precautionary principle, we developed a new sensitive method for the screening of pasteurized milk for pathogenic bacteria. From January 1 to August 31, 2017, 2526 samples of pasteurized milk were prospectively included in the study. We showed that a 20 mL sample of pasteurized milk incubated for 18 h at 37 °C under aerobic conditions was favoring the detection of B. Cereus. The nonconformity rate was 6.3% for the reference method and 12.6% for the improved method (p
Databáze: OpenAIRE