cDNA cloning of a developmentally regulated hemocyanin subunit in the crustacean Cancer magister and phylogenetic analysis of the hemocyanin gene family
| Autor: | N B Terwilliger, G Durstewitz |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA
Complementary Brachyura Protein subunit medicine.medical_treatment Molecular Sequence Data chemical and pharmacologic phenomena Biology Polymerase Chain Reaction Conserved sequence Phylogenetics Complementary DNA Hemolymph Genetics medicine Animals Gene family Amino Acid Sequence Pancreas Molecular Biology Peptide sequence Conserved Sequence Phylogeny Ecology Evolution Behavior and Systematics Binding Sites Base Sequence Sequence Homology Amino Acid Gene Expression Regulation Developmental hemic and immune systems Hemocyanin Sequence Analysis DNA Molecular biology Liver Evolutionary biology Multigene Family Hemocyanins Copper |
| Zdroj: | Molecular Biology and Evolution. 14:266-276 |
| ISSN: | 1537-1719 0737-4038 |
| DOI: | 10.1093/oxfordjournals.molbev.a025762 |
| Popis: | The complete cDNA sequence and protein reading frame of a developmentally regulated hemocyanin subunit in the Dungeness crab (Cancer magister) is presented. The protein sequence is aligned with 18 potentially homologous hemocyanin-type proteins displaying apparent sequence similarities. Functional domains are identified, and a comparison of predicted hydrophilicities, surface probabilities, and regional backbone flexibilities provides evidence for a remarkable degree of structural conservation among the proteins surveyed. Parsimony analysis of the protein sequence alignment identifies four monophyletic groups on the arthropodan branch of the hemocyanin gene tree: crustacean hemocyanins, insect hexamerins, chelicerate hemocyanins, and arthropodan prophenoloxidases. They form a monophyletic group relative to molluscan hemocyanins and nonarthropodan tyrosinases. Arthropodan prophenoloxidases, although functionally similar to tyrosinases, appear to belong to the arthropodan hexamer-type hemolymph proteins as opposed to molluscan hemocyanins and tyrosinases. |
| Databáze: | OpenAIRE |
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