Imaging extracellular potassium dynamics in brain tissue using a potassium-sensitive nanosensor
| Autor: | Jean-Yves Chatton, Joel Wellbourne-Wood, Theresa S. Rimmele |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Fluorescence-lifetime imaging microscopy Radiological and Ultrasound Technology Chemistry Neuroscience (miscellaneous) Nanotechnology Brain tissue Research Papers 03 medical and health sciences Microelectrode extracellular space fluorescence microscopy fluorescent indicator nanotechnology potassium potassium buffering two-photon imaging 030104 developmental biology 0302 clinical medicine Nanosensor Extracellular Biophysics Molecule Radiology Nuclear Medicine and imaging Extracellular potassium Extracellular field potential 030217 neurology & neurosurgery |
| Zdroj: | Neurophotonics, vol. 4, no. 1, pp. 015002 |
| Popis: | Neuronal activity results in the release of [Formula: see text] into the extracellular space (ECS). Classically, measurements of extracellular [Formula: see text] ([Formula: see text]) are carried out using [Formula: see text]-sensitive microelectrodes, which provide a single point measurement with undefined spatial resolution. An imaging approach would enable the spatiotemporal mapping of [Formula: see text]. Here, we report on the design and characterization of a fluorescence imaging-based [Formula: see text]-sensitive nanosensor for the ECS based on dendrimer nanotechnology. Spectral characterization, sensitivity, and selectivity of the nanosensor were assessed by spectrofluorimetry, as well as in both wide-field and two-photon microscopy settings, demonstrating the nanosensor efficacy over the physiologically relevant ion concentration range. Spatial and temporal kinetics of the nanosensor responses were assessed using a localized iontophoretic [Formula: see text] application on a two-photon imaging setup. Using acute mouse brain slices, we demonstrate that the nanosensor is retained in the ECS for extended periods of time. In addition, we present a ratiometric version of the nanosensor, validate its sensitivity in brain tissue in response to elicited neuronal activity and correlate the responses to the extracellular field potential. Together, this study demonstrates the efficacy of the [Formula: see text]-sensitive nanosensor approach and validates the possibility of creating multimodal nanosensors. |
| Databáze: | OpenAIRE |
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