Pregnancy-associated changes in expression of progesterone receptor and progesterone-induced blocking factor genes in bone marrow of ewes
| Autor: | Hao Mi, Jin-Kun Yan, Ling Yang, Le-Ying Zhang, Xian-Xi Yan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Pregnancy Proteins Biology 03 medical and health sciences 0302 clinical medicine Endocrinology Immune system Food Animals Western blot Bone Marrow Pregnancy Internal medicine Progesterone receptor Suppressor Factors Immunologic medicine Animals Conceptus RNA Messenger Estrous cycle Sheep 030219 obstetrics & reproductive medicine medicine.diagnostic_test General Medicine 030104 developmental biology Lymphatic system medicine.anatomical_structure Gene Expression Regulation Pregnancy Animal Immunohistochemistry Female Animal Science and Zoology Bone marrow Receptors Progesterone |
| Zdroj: | Animal Reproduction Science. 186:77-84 |
| ISSN: | 0378-4320 |
| Popis: | Progesterone (P4) regulates reproductive and immune functions through binding to the progesterone receptor (PGR), and the effects of P4 are partly mediated by a progesterone-induced blocking factor (PIBF). Bone marrow (BM) is a key component of the lymphatic system and has an important role in immune response. In this study, BM was harvested from femurs on days 13, 16 and 25 of pregnancy and day 16 of the estrous cycles without mated by intact rams, and a qRT-PCR assay, Western blot and an immunohistochemistry analysis were used to analyze the expression of PGR and PIBF genes in BM. The results showed that there was an increase in relative abundance of PGR and PIBF mRNA in BM during early pregnancy, and PGR-B and the full-length PIBF genes were up-regulated in pregnant ewes. Immunohistochemistry results confirmed that the PGR and PIBF proteins were localized in both the cytoplasm and nuclei of adipocytes and the cells in the stroma and capillaries. This is the first study reporting an up-regulated expression of PGR-B and full-length PIBF genes in BM during early pregnancy in sheep. It is suggested that the conceptus exerted its effects on the adipocytes and the cells in the stroma and capillaries in BM, which were involved in the immunoregulation of BM through both cytosolic and nuclear pathways in ewes. |
| Databáze: | OpenAIRE |
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