Expression profile of Fc receptor-like molecules in patients with IgA nephropathy
| Autor: | Zhong Zhong, Qingyu Kong, Zhijian Li, Jianbo Li, Dongying Fu, Dianchun Shi, Mengjiao Xiao, Shaozhen Feng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine Immunology Fc receptor Down-Regulation Receptors Cell Surface urologic and male genital diseases Peripheral blood mononuclear cell Nephropathy Pathogenesis 03 medical and health sciences 0302 clinical medicine Gene expression medicine Humans Immunology and Allergy Clinical significance Receptors Immunologic Cells Cultured biology business.industry Membrane Proteins Glomerulonephritis IGA Glomerulonephritis General Medicine Middle Aged Galactosyltransferases medicine.disease 030104 developmental biology Leukocytes Mononuclear biology.protein Female Transcriptome business C1GALT1 Molecular Chaperones 030215 immunology |
| Zdroj: | Human Immunology. 82:186-192 |
| ISSN: | 0198-8859 |
| DOI: | 10.1016/j.humimm.2021.01.011 |
| Popis: | Background Fc receptor-like (FCRL) molecules were considered to play a role in the pathogenesis of certain autoimmune diseases. Nonetheless, the clinical significance of FCRLs in IgA nephropathy (IgAN) remains unclear. Objective This study is aimed at investigating the expression levels of FCRLs molecules in IgAN patients and determining its relevance to disease activity. Methods The mRNA expression levels of FCRLs were determined in peripheral blood mononuclear cells (PBMCs) of 42 IgAN patients and 48 healthy controls by quantitative real-time PCR (qRT-PCR). FCRLs proteins expression in B cells of 25 IgAN patients, 14 patients with non-IgAN glomerulonephritis, and 29 healthy controls were detected by Flow cytometry. The Spearman correlation test was used to assess the correlation of FCRLs expression with clinical parameters of IgAN patients. Results Our results indicated significant down-regulation of FCRL2 and FCRL3 mRNA levels in IgAN patients compared to healthy subjects. Surface protein expression of FCRLs molecules confirmed the qRT-PCR results. But FCRL2 and FCRL3 protein levels did not correlate with clinicopathologic phenotypes of IgAN patients. However, we found a significant positively correlation of FCRL2 and FCRL3 mRNA expression with the core 1 β1,3-galactosyltransferase (C1GALT1) and its molecular chaperone (Cosmc) mRNA levels in IgAN patients. Conclusions FCRL2 and FCRL3 expression levels in IgAN patients are significantly decreased and correlated with CIGALT1 and Cosmc mRNA expression. |
| Databáze: | OpenAIRE |
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