Human Papilloma Virus 18 Detection in Oral Squamous Cell Carcinoma and Potentially Malignant Lesions Using Saliva Samples
| Autor: | Mannil Thomas Abraham, Nik Mohd Mazuan Nik Mohd Rosdy, Rosnah Binti Zain, Gabriele Ruth Anisah Froemming, Thong Kwai-Lin, Khor Goot-Heah |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Saliva Pathology medicine.medical_specialty Epidemiology viruses Pcr cloning Biology medicine.disease_cause medicine Humans Basal cell Papillomaviridae Human papilloma virus Papillomavirus Infections Public Health Environmental and Occupational Health Cancer medicine.disease stomatognathic diseases Oncology DNA Viral Immunology Mouth Neoplasms Carcinogenesis Nested polymerase chain reaction Viral load |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 13:6109-6113 |
| ISSN: | 1513-7368 |
| Popis: | Background Oral cancer has become one of the most prevalent cancers worldwide and human Papillomavirus is one of the risk factors for developing oral cancer. For this study HPV18 was chosen as it is one of the high risk HPV types and may lead to carcinogenesis. However, prevalence of HPV18 infection in Oral Squamous Cell Carcinoma in Malaysia remains unclear. Objective This study aimed to investigate the viral load of HPV18 DNA in OSCC and potentially malignant lesions using saliva samples. Materials and methods Genomic DNAs of thirty saliva samples of normal subjects and thirty saliva samples compromised of 16 samples from potentially malignant lesions and 14 of OSCC patients were amplified for HPV18 DNA using a nested polymerase chain reaction analysis. All PCR products were then analyzed using the Bioanalyzer to confirm presence of HPV18 DNA. Result From thirty patients examined, only one of 30 (3.3%) cases was found to be positive for HPV18 in this study. Conclusion The finding of this study revealed that there is a low viral detection of HPV18 in Malaysian OSCC by using saliva samples, suggesting that prevalence of HPV18 may not be important in this group of Malaysian OSCC. |
| Databáze: | OpenAIRE |
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