Antioxidant Effect of Melatonin in Human Retinal Neuron Cultures
| Autor: | Young-Taek Chung, Min-Cheol Lee, Hyung-Seok Kim, Jong-Jae Jung, Seung U. Kim, Jae Hyuk Lee |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Xanthine Oxidase Programmed cell death medicine.medical_specialty Cell Survival alpha-Tocopherol Biology Antioxidants Retina Melatonin chemistry.chemical_compound Developmental Neuroscience Internal medicine medicine Humans MTT assay Xanthine oxidase Cells Cultured Hypoxanthine Electrophoresis Agar Gel Neurons Dose-Response Relationship Drug Retinal DNA Neuroprotective Agents medicine.anatomical_structure Endocrinology Neurology chemistry Apoptosis Female Reactive Oxygen Species medicine.drug |
| Zdroj: | Experimental Neurology. 172:407-415 |
| ISSN: | 0014-4886 |
| DOI: | 10.1006/exnr.2001.7793 |
| Popis: | This study investigates whether the neurohormone melatonin can prevent the retinal neuronal injury caused by reactive oxygen species (ROS) in cultured human retinal neuronal cells. Cultures of human retinal neuronal cells established from a variety of donors were grown to 14 days and then subjected to experimental hypoxanthine/xanthine oxidase (HX/XO)-induced injury. Intracellular production of ROS by administration of HX/XO was confirmed by flow cytometry; the ROS resulted in both apoptotic and necrotic pattern of cell death in the retinal neuron cultures. The efficacy of melatonin against ROS injury was quantitated by MTT assay, enzyme immunoassay, and immunocytochemistry for neurofilament protein. The antioxidative effect of melatonin was compared with that of alpha-tocopherol. Retinal neuronal injury significantly reduced in a dose-response manner by a treatment of 1.0-8.0 mM alpha-tocopherol. Melatonin, in concentrations of more than 2.0 mM, also significantly reduced the injury. About 70% of cells are rescued by pretreatment with 1.0 mM alpha-tocopherol and 8.0 mM melatonin in the MTT assay. Our observations suggest that melatonin can rescue retinal neurons from ROS injury in human retinal cell cultures. |
| Databáze: | OpenAIRE |
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