Curcumin Inhibits Immunostimulatory Function of Dendritic Cells: MAPKs and Translocation of NF-κB as Potential Targets
| Autor: | Hee-Jeong Lee, Young Chul Park, Soong-Hwan Lee, Ki Hyung Kim, Yeong-Min Park, Soon-Cheol Ahn, Chang-Min Lee, Man-Soo Yoon, Gi-Young Kim, Dong-Oh Moon |
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| Rok vydání: | 2005 |
| Předmět: |
Lipopolysaccharides
Male Curcumin medicine.medical_treatment Immunology Active Transport Cell Nucleus Dose-Response Relationship Immunologic Bone Marrow Cells Proinflammatory cytokine Mice chemistry.chemical_compound Adjuvants Immunologic MHC class I medicine Animals Immunology and Allergy Phosphorylation Cells Cultured CD86 Mice Inbred BALB C MHC class II biology NF-kappa B Transcription Factor RelA Cell Differentiation Dendritic Cells Th1 Cells Interleukin-12 Endocytosis Growth Inhibitors Interleukin-10 Cell biology Mice Inbred C57BL Protein Transport Cytokine chemistry biology.protein Inflammation Mediators Mitogen-Activated Protein Kinases Signal transduction CD80 |
| Zdroj: | The Journal of Immunology. 174:8116-8124 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.174.12.8116 |
| Popis: | Curcumin has been shown to exhibit anti-inflammatory, antimutagenic, and anticarcinogenic activities. However, the effect of curcumin on the maturation and immunostimulatory function of dendritic cells (DC) largely remains unknown. In this study, we examined whether curcumin can influence surface molecule expression, cytokine production, and their underlying signaling pathways in murine bone marrow-derived DC. DC were derived from murine bone marrow cells and used as immature or LPS-stimulated mature cells. The DC were tested for surface molecule expression, cytokine production, dextran uptake, the capacity to induce T cell differentiation, and their underlying signaling pathways. Curcumin significantly suppressed CD80, CD86, and MHC class II expression, but not MHC class I expression, in the DC. The DC also exhibited impaired IL-12 expression and proinflammatory cytokine production (IL-1β, IL-6, and TNF-α). The curcumin-treated DC were highly efficient at Ag capture, via mannose receptor-mediated endocytosis. Curcumin inhibited LPS-induced MAPK activation and the translocation of NF-κB p65. In addition, the curcumin-treated DC showed an impaired induction of Th1 responses and a normal cell-mediated immune response. These novel findings provide new insight into the immunopharmacological role of curcumin in impacting on the DC. These novel findings open perspectives for the understanding of the immunopharmacological role of curcumin and therapeutic adjuvants for DC-related acute and chronic diseases. |
| Databáze: | OpenAIRE |
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