Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load
| Autor: | Pier Giulio Conaldi, Carlo Federico Perno, Aurelia Gaeta, Giulia Piccirilli, P Paba, Francesca Sidoti, Guido Antonelli, Agata Calvario, Fausto Baldanti, Cristina Costa, Maria Rosaria Capobianchi, Isabella Abbate, Maurizio Sanguinetti, Maria Luisa Scarasciulli, Giuseppe Sodano, Tiziana Lazzarotto, Luisa Barzon, Antonio Piralla, Maria Linda Vatteroni, Rosaria Santangelo, Giorgio Palù, Rossana Cavallo |
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| Přispěvatelé: | Sidoti F, Piralla A, Costa C, Scarasciulli ML, Calvario A, Conaldi PG, Paba P, Perno CF, Gaeta A, Antonelli G, Sodano G, Santangelo R, Sanguinetti M, Vatteroni ML, Barzon L, Palù G, Abbate I, Capobianchi MR, Piccirilli G, Lazzarotto T, Baldanti F, Cavallo R. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Normalization (statistics) Dna load Quantification methods HCMV International standard Multicenter study viruses 030106 microbiology Cytomegalovirus Biology World Health Organization Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA 03 medical and health sciences 0302 clinical medicine Humans 030212 general & internal medicine Reference standards Whole blood Plasma samples Reproducibility of Results General Medicine Nucleic acid amplification technique Reference Standards Viral Load Molecular biology Infectious Diseases Cytomegalovirus Infections DNA Viral International standard HCMV Multicenter study Nucleic Acid Amplification Techniques |
| Popis: | The present multicentric (n = 11 laboratories) study aimed to identify conversion factors from copies/mL to international units (IU)/mL for the normalization of HCMV DNA load using the first WHO International Standard for HCMV nucleic acid amplification techniques and to enhance interlaboratory agreement of HCMV DNA quantification methods. Study protocols for whole blood and plasma (extraction and amplification) were performed to calculate conversion factors from HCMV DNA copy number to IU. The greatest variability was observed in samples with lower HCMV concentrations (3.0 Log10) in both biological matrices. Overall, 73.1% (206/282) of whole blood and 82.2% (324/394) of plasma samples analyzed fell within an acceptable variation range (±0.5 Log10 difference). An average of 0.64 (range 0.21–1.17) was the conversion factor calculated for the HCMV whole blood panel and 0.82 (range 0.39–2.2) for the HCMV plasma panel. |
| Databáze: | OpenAIRE |
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