Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level
| Autor: | Sara M. Rankin, Amin Garbout, Silvia A. Ferreira, Andrew A. Pitsillides, Ulrich Hansen, Jeffrey N. Clark, Behzad Javaheri, Jonathan R.T. Jeffers |
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| Přispěvatelé: | Engineering & Physical Science Research Council (EPSRC) |
| Rok vydání: | 2020 |
| Předmět: |
musculoskeletal diseases
Cartilage Articular Micro-CT 0301 basic medicine Materials science Population Biomedical Engineering Contrast Media Articular cartilage Osteoarthritis Chondrocyte Imaging 03 medical and health sciences Chondrocytes Imaging Three-Dimensional 0302 clinical medicine 0903 Biomedical Engineering Rheumatology Contrast-to-noise ratio medicine Animals Microscopy Phase-Contrast Orthopedics and Sports Medicine education 030203 arthritis & rheumatology education.field_of_study Micro computed tomography 1103 Clinical Sciences Histology X-Ray Microtomography musculoskeletal system medicine.disease Arthritis & Rheumatology 030104 developmental biology medicine.anatomical_structure Cattle Tomography 3D Biomedical engineering |
| Zdroj: | OSTEOARTHRITIS AND CARTILAGE |
| ISSN: | 1063-4584 |
| DOI: | 10.1016/j.joca.2019.10.007 |
| Popis: | Objective High-resolution non-invasive three-dimensional (3D) imaging of chondrocytes in articular cartilage remains elusive. The aim of this study was to explore whether laboratory micro-computed tomography (micro-CT) permits imaging cells within articular cartilage. Design Bovine osteochondral plugs were prepared four ways: in phosphate-buffered saline (PBS) or 70% ethanol (EtOH), both with or without phosphotungstic acid (PTA) staining. Specimens were imaged with micro-CT following two protocols: 1) absorption contrast (AC) imaging 2) propagation phase-contrast (PPC) imaging. All samples were scanned in liquid. The contrast to noise ratio (C/N) of cellular features quantified scan quality and were statistically analysed. Cellular features resolved by micro-CT were validated by standard histology. Results The highest quality images were obtained using propagation phase-contrast imaging and PTA-staining in 70% EtOH. Cellular features were also visualised when stained in PBS and unstained in EtOH. Under all conditions PPC resulted in greater contrast than AC (p < 0.0001 to p = 0.038). Simultaneous imaging of cartilage and subchondral bone did not impede image quality. Corresponding features were located in both histology and micro-CT and followed the same distribution with similar density and roundness values. Conclusions Three-dimensional visualisation and quantification of the chondrocyte population within articular cartilage can be achieved across a field of view of several millimetres using laboratory-based micro-CT. The ability to map chondrocytes in 3D opens possibilities for research in fields from skeletal development through to medical device design and treatment of cartilage degeneration. |
| Databáze: | OpenAIRE |
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