Studies on inactivation of pathogenic microorganisms in culture media and in bovine semen by photosensitive agents
| Autor: | H.L. Ruhnke, W. C. D. Hare, A. Bielanski, M. D. Eaglesome |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Radiation-Sensitizing Agents Light Semen Biology Ureaplasma Microbiology Virus Tissue culture chemistry.chemical_compound Campylobacter fetus Mycoplasma Ureaplasma diversum Animals Pyronine Hematoporphyrin Derivative Herpesvirus 1 Bovine Leptospira Hematoporphyrin Diarrhea Viruses Bovine Viral General Veterinary General Medicine biology.organism_classification Virology Sperm Culture Media Hematoporphyrins chemistry Cattle Mycoplasma bovigenitalium |
| Zdroj: | Veterinary Microbiology. 38:277-284 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/0378-1135(94)90008-6 |
| Popis: | The application of three photosensitive agents for disinfection of bovine semen was investigated. Bovine microbial pathogens suspended in tissue culture medium and/or PBS and also added to bovine semen were exposed to the photosensitive agents followed by irradiation. Hematoporphyrin, hematoporphyrin derivative and thiopyronine were effective against bovine herpes virus-1, bovine viral diarrhoea virus, Mycoplasma bovigenitalium, Mycoplasma canadense, and Ureaplasma diversum in culture media. In addition, thiopyronine was effective against Leptospira pomona. Similar treatments were not effective against Leptospira hardjo, Mycoplasma bovis, or Campylobacter fetus subsp. venerealis. When microorganisms were added to bovine semen, only bovine herpes virus-1 was controlled by the photosensitive agents used at concentrations which did not appear harmful to sperm cells. |
| Databáze: | OpenAIRE |
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