Proinflammatory effects of diesel exhaust particles from moderate blend concentrations of 1st and 2nd generation biodiesel in BEAS-2B bronchial epithelial cells—The FuelHealth project
| Autor: | Remigiusz Mruk, Joanna Gromadzka-Ostrowska, Anna Lankoff, Pål Magnusson, Magne Refsnes, Michał Oczkowski, Johan Øvrevik, Tonje Skuland, Oddvar Myhre, Marcin Kruszewski |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Diesel exhaust Health Toxicology and Mutagenesis Gene Expression Bronchi 010501 environmental sciences Toxicology complex mixtures 01 natural sciences Cell Line Proinflammatory cytokine 03 medical and health sciences Diesel fuel chemistry.chemical_compound Cytochrome P-450 CYP1A1 Humans Food science Fatty acid methyl ester Vehicle Emissions 0105 earth and related environmental sciences Pharmacology chemistry.chemical_classification Air Pollutants Biodiesel Interleukin-6 Chemistry Interleukin-8 food and beverages Epithelial Cells General Medicine 030104 developmental biology Vegetable oil Hydrocarbon Biofuel Biofuels Particulate Matter Heme Oxygenase-1 |
| Zdroj: | Environmental Toxicology and Pharmacology. 52:138-142 |
| ISSN: | 1382-6689 |
| DOI: | 10.1016/j.etap.2017.04.004 |
| Popis: | Biodiesel fuel fuels are introduced at an increasing extent as a more carbon-neutral alternative to reduce CO2-emissions, compared to conventional diesel fuel. In the present study we have investigated the impact of increasing the use of 1st generation fatty acid methyl ester (FAME) biodiesel from current 7% blend (B7) to 20% blend (B20), or by increasing the biodiesel content by adding 2nd generation hydrotreated vegetable oil (HVO) based biodiesel (SHB; Synthetic Hydrocarbon Biofuel) on toxicity of diesel exhaust particles (DEP) in an in vitro system. Human bronchial epithelial BEAS-2B cells were exposed for 4 and 20h to DEP from B7, B20 and SHB at different concentrations, and examined for effects on gene expression of interleukin 6 (IL-6), CXCL8 (IL-8), CYP1A1 and heme oxygenase-1 (HO-1). The results show that both B20 and SHB were more potent inducers of IL-6 expression compared to B7. Only B20 induced statistically significant increases in CXCL8 expression. By comparison the rank order of potency to induce CYP1A1 was SHB>B7>B20. No statistically significant difference were observed form HO-1 expression, suggesting that the differences in cytokine responses were not due to oxidative stress. The results show that even moderate increases in biodiesel blends, from 7% to 20%, may increase the proinflammatory potential of emitted DEP in BEAS-2B cells. This effect was observed for both addition of 1st generation FAME and 2nd generation HVO biodiesel. |
| Databáze: | OpenAIRE |
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