Concurrent Prebiotic Formation of Nucleoside‐Amidophosphates and Nucleoside‐Triphosphates Potentiates Transition from Abiotic to Biotic Polymerization

Autor:	Eddy I. Jiménez, Ramanarayanan Krishnamurthy, Huacan Lin, Joshua T Arriola, Ulrich Müller
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Oligonucleotide RNA Nucleosides General Medicine General Chemistry Combinatorial chemistry Catalysis Phosphates Polymerization chemistry.chemical_compound Enzyme chemistry Nucleic acid Phosphorylation Nucleoside DNA
Zdroj:	Angewandte Chemie International Edition. 61
ISSN:	1521-3773 1433-7851
Popis:	Polymerization of nucleic acids in biology utilizes 5'-nucleoside triphosphates (NTPs) as substrates. The prebiotic availability of NTPs has been unresolved and other derivatives of nucleoside-monophosphates (NMPs) have been studied. However, this latter approach necessitates a change in chemistries when transitioning to biology. Herein we show that diamidophosphate (DAP), in a one-pot amidophosphorylation-hydrolysis setting converts NMPs into the corresponding NTPs via 5'-nucleoside amidophosphates (NaPs). The resulting crude mixture of NTPs are accepted by proteinaceous- and ribozyme-polymerases as substrates for nucleic acid polymerization. This phosphorylation also operates at the level of oligonucleotides enabling ribozyme-mediated ligation. This one-pot protocol for simultaneous generation of NaPs and NTPs suggests that the transition from prebiotic-phosphorylation and oligomerization to an enzymatic processive-polymerization can be more continuous than previously anticipated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b549edcbdf8eff34643fdf83613e9d10 https://doi.org/10.1002/anie.202113625 Zobrazit plný text záznamu Plný text