Effect of Nano-Platinum on Proliferation and Apoptosis of Non-Small Cell Lung Cancer Cells via P53 Pathway
| Autor: | Yiqun Liu, Long Zhao, Fangjuan Hu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lung Neoplasms
Materials science Biomedical Engineering Antineoplastic Agents Apoptosis Bioengineering 030226 pharmacology & pharmacy 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung Cell Line Tumor medicine Humans General Materials Science Lung cancer Cell Proliferation Platinum Cisplatin A549 cell Cell growth General Chemistry respiratory system Condensed Matter Physics medicine.disease 030210 environmental & occupational health respiratory tract diseases Drug Resistance Neoplasm Cytoplasm Cell culture Cancer research Nanoparticles Tumor Suppressor Protein p53 Signal transduction medicine.drug |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 21:903-908 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2021.18629 |
| Popis: | This paper studies the non-small cell lung cancer with interventional treatment of polyethylene imine-coated platinum nanoclusters. The relationship between the inhibition of non-small cell lung cancer cell proliferation, apoptosis, and the expression of its related apoptotic protein p53. Apoptosis experiments were applied was used to detect the effect of nanoplatin on A549 cells. Western blot was used to detect the expression of p53 protein in A549 cells after nanoplatin treatment. We co-cultured nanoplatin with cells for 4 hours and performed nuclear staining. Observed by laser confocal microscopy, we found that nanoplatin can enter the nucleus and cytoplasm of non-small cell lung cancer A549 cells, and the phenomenon of drug entering the nucleus is very obvious. Treatment of non-small cell lung cancer with A549 cells at different concentrations of nanoplatin for 24 hours, with the increase of the concentration, the proliferation inhibition rate of non-small cell lung cancer gradually increased. Treatment of cisplatin-resistant non-small cell lung cancer cells with different concentrations of nanoplatin for 24 hours, as the concentration increases, the proliferation inhibition rate of non-small cell lung cancer gradually increases. After treatment of A549 cells with different concentrations of nanoplatin for 24 hours, the apoptosis rate of A549 cells gradually increased with the increase of drug concentration. Finally, we conclude that nanoplatin has not only inhibitory and pro-apoptotic effects on non-small cell lung cancer cells, but also fluorescent labeling. Compared with cisplatin, nanoplatin can significantly inhibit the proliferation of non-small cell lung cancer cells and overcome its cisplatin resistance. The p53-related signaling pathway may participate in the process of apoptosis and reverse the process of cisplatin resistance, but the specific mechanism and whether there are other signaling pathways involved Further research is needed. |
| Databáze: | OpenAIRE |
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