The effect of salt stress on the production of apocarotenoids and the expression of genes related to their biosynthesis in saffron
| Autor: | Golandam Sharifi, Fateme Sadat Moslemi, Atousa Vaziri, Javad Gharechahi |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
food.ingredient ved/biology.organism_classification_rank.species Sodium Chloride Real-Time Polymerase Chain Reaction Salt Stress Crocin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food Glucosides Gene Expression Regulation Plant Crocus sativus Cyclohexenes Genetics Food science Molecular Biology Carotenoid Flavor Chromatography High Pressure Liquid chemistry.chemical_classification ved/biology Terpenes Food additive Sodium General Medicine Metabolism Crocus Picrocrocin Carotenoids Safranal Biosynthetic Pathways Plant Leaves 030104 developmental biology chemistry 030220 oncology & carcinogenesis Potassium |
| Zdroj: | Molecular biology reports. 48(2) |
| ISSN: | 1573-4978 |
| Popis: | Saffron stigmas are widely used as food additives and as traditional medicine in Iran and many other countries. The unique taste, flavor and pharmaceutical properties of saffron stigmas are due to the presence of three apocarotenoids secondary metabolites crocin, picrocrocin and safranal. There is limited knowledge about the effect of environmental stresses on the metabolism of apocarotenoids in saffron. We analyzed the content of crocin and picrocrocin and the expression of key genes of apocarotenoid biosynthesis pathways (CsCCD2, CsCCD4, CsUGT2, CsCHY-β and CsLCYB) in saffron plants exposed to moderate (90 mM) and high (150 mM) salt (NaCl) concentrations. Measuring ion concentrations in leaves showed an increased accumulation of Na+ and decreased uptake of K+ in salt treated compared to control plants indicating an effective salt stress. HPLC analysis of apocarotenoids revealed that crocin production was significantly halted (P |
| Databáze: | OpenAIRE |
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