Differences in Chlamydia trachomatis growth rates in human keratinocytes among lymphogranuloma venereum reference strains and clinical isolates
| Autor: | Bronwyn C. Joubert, A. Willem Sturm |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Keratinocytes
Microbiology (medical) Serotype Chlamydia trachomatis Human skin Biology urologic and male genital diseases medicine.disease_cause Microbiology Statistics Nonparametric Cell Line Pathogenesis medicine Humans Cell Proliferation Strain (chemistry) Lymphogranuloma venereum Skin Diseases Bacterial General Medicine medicine.disease Virology female genital diseases and pregnancy complications On cells Microscopy Fluorescence Skin cell Lymphogranuloma Venereum |
| Zdroj: | Journal of Medical Microbiology. 60:1565-1569 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.032169-0 |
| Popis: | The pathogenesis of the primary stage of lymphogranuloma venereum (LGV) is poorly understood. There is no skin cell model and LGV pathogenesis studies are therefore carried out on cells of different origin. Moreover, such studies usually use reference strains, which may have evolved over the years in culture. In this study, a model was developed in which Chlamydia trachomatis enters and grows in human keratinocytes at 37 and 33 °C. Keratinocytes were infected with fresh clinical isolates and the three LGV reference strains L1, L2 and L3. Growth was monitored for 5 days post-infection using fluorescence microscopy and image analysis software. Chlamydial replication was quicker at 37 than at 33 °C, despite 33 °C being the temperature of human skin. The serovar L2 reference strain grew significantly faster than the other strains, although the fresh clinical isolates were also serovar L2. When grown in keratinocytes at 33 °C, the L2 and L3 reference strains produced much larger inclusions than the other strains tested. This model, which utilizes keratinocytes, better simulates the conditions present at the initial site of infection in LGV than previously published literature, making it a useful tool for future LGV pathogenesis studies. In addition, the results indicate that fresh clinical isolates should be included in LGV pathogenesis studies. |
| Databáze: | OpenAIRE |
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