Efficacy of Low Molecular Heparin on Preeclampsia by Inhibiting Apoptosis of Trophoblasts via the p38MAPK Signaling Pathway

Autor: Manzhen Zuo, Dandan Quan, Li Li
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Apoptosis
p38 Mitogen-Activated Protein Kinases
0302 clinical medicine
Pre-Eclampsia
Cell Movement
Pregnancy
reproductive and urinary physiology
030219 obstetrics & reproductive medicine
Chemistry
Applied Mathematics
General Medicine
Heparin
Trophoblasts
Treatment Outcome
medicine.anatomical_structure
Reperfusion Injury
Modeling and Simulation
embryonic structures
Female
Chorionic Villi
medicine.symptom
Signal transduction
psychological phenomena and processes
Research Article
medicine.drug
medicine.medical_specialty
Article Subject
MAP Kinase Signaling System
education
Computer applications to medicine. Medical informatics
R858-859.7
In Vitro Techniques
behavioral disciplines and activities
General Biochemistry
Genetics and Molecular Biology
Cell Line
Preeclampsia
03 medical and health sciences
Internal medicine
Placenta
medicine
Animals
Humans
Rats
Wistar
Cell Proliferation
Fetus
General Immunology and Microbiology
Activator (genetics)
Computational Biology
Heparin
Low-Molecular-Weight
Hypoxia (medical)
medicine.disease
Rats
Disease Models
Animal
030104 developmental biology
Endocrinology
Zdroj: Computational and Mathematical Methods in Medicine, Vol 2021 (2021)
Computational and Mathematical Methods in Medicine
ISSN: 1748-6718
Popis: Objective. To explore the efficacy of low molecular heparin on preeclampsia by inhibiting apoptosis of trophoblasts via the p38MAPK signaling pathway. Methods. A preeclampsia rat model was established, and the effects of low molecular heparin on preeclampsia via the p38MAPK signaling pathway were analyzed based on intervention of the rats with different combinations of low molecular heparin and p38MAPK signaling pathway activator. Furthermore, a hypoxia/reoxygenation model of trophoblasts in vitro was established to explore the effects of low molecular heparin on trophoblasts via the p38MAPK signaling pathway. Results. After treatment with low molecular heparin, pregnant rats in the heparin group showed significantly decreased blood pressure, 24 h proteinuria, and p38MAPK protein levels in placenta tissues and decreased apoptosis rate of placenta tissue cells (all P < 0.05 ) and showed more fetal rats and lowered weight of them (both P < 0.05 ) but showed no significant change in the weight of placenta (all P > 0.05 ). Pregnant rats treated with low molecular heparin and p38MAPK activator showed significantly higher blood pressure, 24 h proteinuria, and p38MAPK protein levels in placenta tissues and apoptosis rate of placenta tissue cells than those of pregnant rats in the heparin group (all P < 0.05 ) and also showed less fetal rats and lighter fetal rats than those in the heparin group (both P < 0.05 ) but showed no difference with them in the weight of placenta ( P > 0.05 ). Further analysis revealed that low molecular heparin could protect the survival and migration of trophoblasts under hypoxia/reoxygenation conditions and reduce apoptosis of them (all P < 0.05 ). Conclusion. Low molecular heparin can alleviate preeclampsia by inhibiting the p38MAPK signaling pathway and can inhibit apoptosis of trophoblasts and promote proliferation and migration of them.
Databáze: OpenAIRE
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