Type I restriction enzyme with RecA protein promotes illegitimate recombination
| Autor: | Ichizo Kobayashi, Kohji Kusano, Masaru Tanokura, Yasuo Asami, Ayumi Fujita |
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| Rok vydání: | 2003 |
| Předmět: |
Recombination
Genetic Genetics Base Sequence Models Genetic Inverted repeat Escherichia coli Proteins FLP-FRT recombination Molecular Sequence Data Deoxyribonucleases Type I Site-Specific Non-allelic homologous recombination DNA Restriction Enzymes Biology Genetic recombination Non-homologous end joining Rec A Recombinases Restriction enzyme Sequence Homology Nucleic Acid Escherichia coli Site-specific recombination Homologous recombination Molecular Biology Plasmids Repetitive Sequences Nucleic Acid |
| Zdroj: | Plasmid. 50:202-212 |
| ISSN: | 0147-619X |
| Popis: | Illegitimate (non-homologous) recombination requires little or no sequence homology between recombining DNAs and has been regarded as being a process distinct from homologous recombination, which requires a long stretch of homology between recombining DNAs. However, we have found a type of illegitimate recombination that requires an interaction between long homologous DNA sequences. It was detected when a plasmid that carried 2-kb-long inverted repeats was subjected to type I (EcoKI) restriction in vivo within a special mutant strain of Escherichia coli. In the present work, we analyzed genetic requirements for this type of illegitimate recombination in well-defined genetic backgrounds. Our analysis demonstrated dependence on RecA function and on the presence of two EcoKI sites on the substrate DNA. These results are in harmony with a model in which EcoKI restriction enzyme attacks an intermediate of homologous recombination to divert it to illegitimate recombination. |
| Databáze: | OpenAIRE |
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