Chronic partial urethral obstruction in female rats: description of an experimental model and initial results
| Autor: | Marcelo Ferreira Cassini, Carlos A F Molina, Antonio Carlos Pereira Martins, Murilo Ferreira de Andrade, Silvio Tucci, Gilson José de Lima |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Urethral Obstruction medicine.medical_treatment Urinary Bladder Distension Muscle hypertrophy Sex Factors Laparotomy medicine Animals Postoperative Period Rats Wistar Obstrução Uretral Hydronephrosis Ratos Urinary bladder business.industry Urinary Bladder Diseases Bexiga Urinária Reproducibility of Results medicine.disease Obstructive Nephropathy Surgery Rats Disease Models Animal medicine.anatomical_structure Urethra Vagina Female business |
| Zdroj: | Acta Cirúrgica Brasileira v.26 suppl.2 2011 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Volume: 26 Supplement 2, Pages: 111-114, Published: 2011 |
| ISSN: | 1678-2674 |
| Popis: | PURPOSE: To develop an experimental model of infravesical urinary obstruction in female rats. METHODS: After median caudal laparotomy, the urethra of 14 female rats was delicately separated from the vagina and loosely wrapped with cellophane tape measuring 0.4 x 1.0 cm. The animals were evaluated 4 (n=7) and 8 (n=7) weeks later. Five additional control animals were only subjected to separation of the urethra and vagina and monitored for 12 weeks. RESULTS: After four weeks, three rats presented vesical dilation associated with discrete ureteral ectasis in 2 animals, with the third presenting discrete hydronephrosis in one kidney. After eight weeks, five rats (71.4%) presented vesical distension with bilateral ureterohydronephrosis. No significant changes (p>0.05) in serum urea or creatinine occurred in any group compared to preoperative values. CONCLUSION: We present here an inexpensive and simple method for the slow induction of urethral obstruction in adult female rats, with the development of progressive vesical hypertrophy and ureterohydronephrosis, which may be used as an experimental model for the study of different aspects of obstructive nephropathy. OBJETIVO: Desenvolver modelo experimental de obstrução urinária infravesical em ratas. MÉTODOS: Após laparotomia caudal mediana, as uretras de 14 ratas foram delicadamente separadas da vagina e frouxamente envoltas com fita de celofane medindo 0.4 x 1.0 cm. Os animais foram avaliados 4 (n=7) e 8 (n=7) semanas depois. Cinco animais adicionais (controle) foram submetidos apenas à separação da uretra e da vagina e monitoradas por 12 semanas. RESULTADOS: Após quatro semanas, três ratas apresentaram dilatação vesical associada a discreta ectasia ureteral em 2 animais, com o terceiro apresentando discreta hidronefrose em um rim. Após oito semanas, cinco ratas (71.4%) apresentaram distensão vesical com ureterohidronefrose bilateral. Não ocorreram alterações significativas (p>0.05) nos valores de uréia ou creatinina em qualquer grupo, comparado com valores pré-operatórios. CONCLUSÃO: Apresentamos aqui um método barato e simples para a indução lenta de obstrução uretral em ratas adultas, com desenvolvimento progressivo de hipertrofia vesical e ureterohidronefrose, podendo ser utilizado como modelo experimental para estudo de diferentes aspectos da nefropatia obstrutiva. |
| Databáze: | OpenAIRE |
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