Cytotoxic T Lymphocytes Derived from Patients with Chronic Hepatitis C Virus Infection Kill Bystander Cells via Fas-FasL Interaction
| Autor: | Jürg Reichen, Andreas Cerny, Darius Moradpour, Benno Grabscheid, Werner J. Pichler, Benno Wölk, Christel Gremion |
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| Rok vydání: | 2004 |
| Předmět: |
Cytotoxicity
Immunologic Fas Ligand Protein Hepatitis C virus Immunology Antigen presentation Apoptosis Hepacivirus Biology medicine.disease_cause Microbiology Fas ligand Virus Cell Line Mice Viral Proteins Antigen Virology medicine Bystander effect Animals Humans Cytotoxic T cell fas Receptor Membrane Glycoproteins Bystander Effect Hepatitis C Chronic Insect Science Hepatocytes Pathogenesis and Immunity T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Virology. 78:2152-2157 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.78.4.2152-2157.2004 |
| Popis: | The role of Fas-mediated lysis of hepatocytes in hepatitis C virus (HCV)-induced injury is frequently discussed. We therefore analyzed the effect of the number of HCV antigen-expressing cells, the mode of antigen presentation, and the number of cytotoxic T lymphocytes in a coculture system mimicking cellular components of the liver. Here, we show that endogenously processed HCV proteins are capable of inducing bystander killing. We further demonstrate that 0.8 to 1.5% of cells presenting HCV antigens suffice to induce lysis of 10 to 29% of bystander cells, suggesting that the mechanism may be operative at low fractions of infected versus uninfected hepatocytes in vivo. Our data underscore the role of the Fas pathway in HCV-related liver injury and support the exploration of Fas-based treatment strategies for patients with chronic hepatitis C virus infection. |
| Databáze: | OpenAIRE |
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