LASER MICRODISSECTION OF PLANT TISSUE: What You See Is What You Get

Autor:	Tie Liu, Neeru Gandotra, S Lori Tausta, Timothy Nelson
Rok vydání:	2006
Předmět:	Cell type Reporter gene Physiology Lasers Cell Biology Plant Science Plants Biology Proteomics Plant cell Molecular biology Chromosomes Plant Staining chemistry.chemical_compound chemistry Plant Cells RNA Messenger Molecular Biology Microdissection DNA Laser capture microdissection
Zdroj:	Annual Review of Plant Biology. 57:181-201
ISSN:	1545-2123 1543-5008
Popis:	Laser microdissection (LM) utilizes a cutting or harvesting laser to isolate specific cells from histological sections; the process is guided by microscopy. This provides a means of removing selected cells from complex tissues, based only on their identification by microscopic appearance, location, or staining properties (e.g., immunohistochemistry, reporter gene expression, etc.). Cells isolated by LM can be a source of cell-specific DNA, RNA, protein or metabolites for subsequent evaluation of DNA modifications, transcript/protein/metabolite profiling, or other cell-specific properties that would be averaged with those of neighboring cell types during analysis of undissected complex tissues. Plants are particularly amenable to the application of LM; the highly regular tissue organization and stable cell walls of plants facilitate the visual identification of most cell types even in unstained tissue sections. Plant cells isolated by LM have been the starting point for a variety of genomic and metabolite studies of specific cell types.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b53cf5eb51fe55830c635e964326cab5 https://doi.org/10.1146/annurev.arplant.56.032604.144138 Zobrazit plný text záznamu