Interacting Factors That Influence Long-term Storage of Live Pasteurella tularensis Vaccine and Rift Valley Fever Virus
| Autor: | Richard C. Carter, Bill G. Mahlandt, Francis J. Weirether, Jerry S. Walker, Michael D. Orlando, Ralph E. Lincoln, Frederick Klein |
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| Rok vydání: | 1969 |
| Předmět: |
Veterinary medicine
Rift Valley Fever Guinea Pigs Preservation Biological General Biochemistry Genetics and Molecular Biology Virus Microbiology Mice Freezing medicine Animals General Pharmacology Toxicology and Pharmaceutics Rift Valley fever Francisella tularensis Attenuated vaccine Pasteurella tularensis General Immunology and Microbiology biology Temperature General Medicine Hydrogen-Ion Concentration biology.organism_classification medicine.disease Culture Media Bacterial vaccine Cell culture Bacterial Vaccines Clinical Microbiology Virology and Immunology Arboviruses Bacteria |
| Zdroj: | Applied Microbiology. 17:427-434 |
| ISSN: | 0003-6919 |
| DOI: | 10.1128/am.17.3.427-434.1969 |
| Popis: | Studies were conducted on the interaction of various parameters which affect the storage stability and growth potential of liquid cultures of Pasteurella tularensis live vaccine strain (LVS) and Rift Valley fever virus Van Wyk strain (RVFV). Storage variables studied with LVS included four storage temperatures (4, -20, -65, -175 C), single and multiple freeze-thaw cycles, two freezing and two thawing rates (slow and fast), various inoculum levels (1, 3, 5, and 10%) for the determination of growth potential, and the retention of immunizing potential (mice and guinea pig) after storage. Neither the freezing rate nor the number of freeze-thaw cycles seriously affected the growth of LVS after storage at -175C; however, the slow rate of thaw proved deleterious as were all temperatures of storage except -175 C after 1 year of storage, as shown by both criteria of evaluation. RVFV produced in two combinations of cell lines and media (LM cell line-199 peptone medium and LDR cell line-Eagle's minimum essential medium) was stored at three serum levels (10, 20, 40%), three p H values (6.2., 7.0, 7.8), and three temperatures (-20, -65, -175 C). These studies indicated: (i) virus produced in the LDR cell line and Eagle's medium was more stable than that produced in the LM cell line and 199 peptone medium for either short- or long-term storage; (ii) serum levels did not affect stability; and (iii) low p H resulted in losses during long-term storage under all conditions tested. Thus, cryogenic storage is advantageous for stock culture maintenance of bacteria and viruses and for other similar applications. |
| Databáze: | OpenAIRE |
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