Characterization of perinatally born glutamatergic neurons of the mouse olfactory bulb based on NeuroD6 expression reveals their resistance to sensory deprivation

Autor: Alexandra Angelova, Nathalie Coré, Jean-Claude Platel, Harold Cremer, Christophe Beclin
Přispěvatelé: Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), Funding Information Agence Nationale de la Recherche. Grant Numbers: ANR‐10‐INBS‐04, ANR‐13‐BSV4‐0013. Fondation A*MIDEX‐ Biotrail program. Association France Parkinson. Fondation de France. Grant Number: FDF70959. Fondation pour la Recherche Médicale. Grant Numbers: FDT20160435597, ING20150532361, ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-13-BSV4-0013,AtmiR,Contrôle moléculaire de la neurogenèse postnatale : génes et microRNAs(2013), ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011)
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
RRID:AB_1587367
Postnatal neurogenesis
Rostral migratory stream
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Receptors
Odorant
Mice
0302 clinical medicine
Genes
Reporter
Basic Helix-Loop-Helix Transcription Factors
RRID:AB_778267
Gene Knock-In Techniques
RRID:IMSR_JAX:007914
RRID:AB_2336063
General Neuroscience
Neurogenesis
RRID:AB_2200219
RRID:AB_2619904
Olfactory Bulb
RRID:SCR_013672
Smell
RRID:AB_828390
RRID:AB_887877
Excitatory postsynaptic potential
GABAergic
Female
Nasal Obstruction
RRID:MGI:4429523
Sensory Receptor Cells
Cell Survival
RRID:AB_10013440
Glutamic Acid
Nerve Tissue Proteins
Biology
Inhibitory postsynaptic potential
Glutamatergic neurons
RRID:MGI:5510844
03 medical and health sciences
Glutamatergic
Animals
Cell Lineage
Sensory deprivation
NeuroD6 bHLH transcription factor
RRID:AB_2068336
RRID:SCR_007370
RRID:AB_2187539
RRID:SCR_001905
RRID:AB_2749806
Olfactory bulb
Mice
Inbred C57BL
RRID:MGI:5308867
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
030104 developmental biology
RRID:AB_393778
Animals
Newborn
nervous system
Sensory Deprivation
RRID:SCR_002285
Neuroscience
030217 neurology & neurosurgery
Zdroj: Journal of Comparative Neurology
Journal of Comparative Neurology, 2019, 527 (7), pp.1245-1260. ⟨10.1002/cne.24621⟩
Journal of Comparative Neurology, John Wiley & Sons, 2019, 527 (7), pp.1245-1260. ⟨10.1002/cne.24621⟩
ISSN: 0021-9967
0092-7317
1550-7130
DOI: 10.1002/cne.24621
Popis: International audience; During postnatal olfactory bulb (OB) neurogenesis, predetermined stem cells residing in the ventricular-subventricular zone continuously generate progenitors that migrate in the rostral migratory stream and integrate into the OB. Although the vast majority of these postnatally generated interneurons are inhibitory, a sub-fraction represents glutamatergic neurons that integrate into the superficial glomerular layer. In the present work, we demonstrate that the bHLH transcription factor NeuroD6 is specifically and transitorily expressed in the dorsal neurogenic lineage that generates glutamatergic juxtaglomerular cells (JGCs) for the OB. Using lineage tracing combined with whole brain clearing, we provide new insight into timing of generation, morphology , and connectivity of glutamatergic JGCs. Specifically, we show that all glutamatergic JGCs send complex axons with varying projection patterns into different layers of the OB. Moreover, we find that, contrary to GABAergic OB interneurons, glutamatergic JGCs survive under sensory deprivation, indicating that inhibitory and excitatory populations are differentially susceptible to environmental stimulation.
Databáze: OpenAIRE
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