| Autor: |
Santillán Martínez, Miguel I., Gao, Dongli, Appiano, Michela, Derks, Inge, Huibers, Robin P., Spil, Glenn, Wang, Xulan, Visser, Richard G. F., Wolters, Anne-Marie A., Bai, Yuling |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Frontiers in Plant Science. 14 |
| ISSN: |
1664-462X |
| Popis: |
To explore specific components of resistance against the tomato-adapted powdery mildew pathogen Pseudoidium neolycopersici (On) in the model plant Arabidopsis, we performed a disease assay in 123 accessions. When testing the resistance in the F1 from crossings between resistant accessions with susceptible Col-0 or Sha, only the progeny of the cross between accession Bla-6 and Col-0 displayed a completely resistant phenotype. The resistance in Bla-6 is known to be specific for Pseudoidium neolycopersici. QTL analysis and fine-mapping through several rounds of recombinant screenings allowed us to locate a major resistance QTL in an interval on chromosome 1, containing two candidate genes and an intergenic insertion. Via CRISPR/Cas9 targeted mutagenesis, we could show that knocking out the ZED-1 RELATED KINASE 13 (ZRK13) gene compromised the On resistance in Bla-6. Several polymorphisms are observed in the ZRK13 allelic variant of Bla-6 when compared to the Col-0 protein. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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