An integrative proteomics method identifies a regulator of translation during stem cell maintenance and differentiation
| Autor: | D. V. Maltseva, Oscar E. Simonson, J. Carlos Villaescusa, Noah Moruzzi, Roman A. Zubarev, Magnus Nordenskjöld, Alexander G. Tonevitsky, Amir Ata Saei, Massimiliano Gaetani, Mike Aoun, Niels Leijten, Kamilya Altynbekova, Vincent Millischer, Rikard Holmdahl, Per Olof Berggren, Pierre Sabatier, Karl-Henrik Grinnemo, Alexander E. Kel, Ana Coelho, Sandeep Kadekar, Sergey Rodin, Christian M. Beusch, Patrick Micke |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pluripotent Stem Cells
Proteomics Cell type Translation Science Cell- och molekylärbiologi Cell General Physics and Astronomy Ribosome biogenesis Stem-cell differentiation Computational biology Biology Proteome informatics Regenerative medicine General Biochemistry Genetics and Molecular Biology Article Cell Line medicine Humans skin and connective tissue diseases Induced pluripotent stem cell Multidisciplinary Clinical Laboratory Medicine Translation (biology) Cell Differentiation General Chemistry Klinisk laboratoriemedicin medicine.anatomical_structure sense organs Stem cell Cell and Molecular Biology |
| Zdroj: | Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021) |
| ISSN: | 2041-1723 |
| Popis: | Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. To systematically study such transitions, we introduce a method that simultaneously measures protein expression and thermal stability changes in cells and provide the web-based visualization tool ProteoTracker. We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. To characterize molecular changes during cell type transitions, the authors develop a method to simultaneously measure protein expression and thermal stability changes. They apply this approach to study differences between human pluripotent stem cells, their progenies, parental and allogeneic cells. |
| Databáze: | OpenAIRE |
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