Critical role of monocytes to support normal B cell and diffuse large B cell lymphoma survival and proliferation

Autor: Charlotte Boix, Thierry Jo Molina, Emilie M. Fournier, Christopher G. Mueller, Cécile Daussy, Wolf H. Fridman, Wing-Hong Kwan
Přispěvatelé: Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunologie et chimie thérapeutiques (ICT), Cancéropôle du Grand Est-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique des Lasers (LPL), Université Paris 13 (UP13)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Sorbonne Paris Cité (USPC)-Institut Galilée-Université Paris 13 (UP13)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Lymphoma
B-Cell
Cell Survival
Immunology
Naive B cell
Lipopolysaccharide Receptors
Enzyme-Linked Immunosorbent Assay
Monocytes
Immunoenzyme Techniques
03 medical and health sciences
0302 clinical medicine
hemic and lymphatic diseases
medicine
Biomarkers
Tumor
Immunology and Allergy
Humans
Myeloid Cells
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
CD40 Antigens
B-cell lymphoma
B-cell activating factor
Chemokine CCL5
B cell
030304 developmental biology
Cell Proliferation
0303 health sciences
B-Lymphocytes
CD40
biology
Cell growth
Reverse Transcriptase Polymerase Chain Reaction
Cell Biology
Dendritic Cells
medicine.disease
Flow Cytometry
Cell biology
B-1 cell
medicine.anatomical_structure
Chemokines
CC
Cancer research
biology.protein
Interleukin-2
Lymph Nodes
Lymphoma
Large B-Cell
Diffuse
Diffuse large B-cell lymphoma
030215 immunology
B-Cell Activation Factor Receptor
Zdroj: Journal of Leukocyte Biology
Journal of Leukocyte Biology, Society for Leukocyte Biology, 2007, 82 (3), pp.567-75 val UPR9021. ⟨10.1189/jlb.0706481⟩
Journal of Leukocyte Biology, 2007, 82 (3), pp.567-75 val UPR9021. ⟨10.1189/jlb.0706481⟩
ISSN: 0741-5400
Popis: Large B cell lymphomas can comprise numerous CD14+ cells in the tumor stroma, which raises the question of whether monocytes can support B cell survival and proliferation. We show that the coculture of monocytes with B cells from peripheral blood or from diffuse large B cell lymphoma enabled prolonged B cell survival. Under these conditions, diffuse large lymphoma B cells proliferated, and addition of B cell-activating factor of the TNF family (BAFF) and IL-2 enhanced cell division. Monocytes and dendritic cells (DC) had similar antiapoptotic activity on healthy B cells but displayed differences with respect to B cell proliferation. Monocytes and cord blood-derived CD14+ cells promoted B cell proliferation in the presence of an anti-CD40 stimulus, whereas DC supported B cell proliferation when activated through the BCR. DC and CD14+ cells were able to induce plasmocyte differentiation. When B cells were activated via the BCR or CD40, they released the leukocyte attractant CCL5, and this chemokine is one of the main chemokines expressed in diffuse large B cell lymphoma. The data support the notion that large B cell lymphoma recruit monocytes via CCL5 to support B cell survival and proliferation.
Databáze: OpenAIRE
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