Phenotypic profile and functional characterization of rat lymph node-derived gammadelta T cells: implication in the immune response to cytomegalovirus
| Autor: | Larissa Dyugovskaya, Mark Hirsh, Haim Ginsburg |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Interleukin 2
Cytotoxicity Immunologic Immunology chemical and pharmacologic phenomena Biology Immunophenotyping Rats Sprague-Dawley Immune system T-Lymphocyte Subsets medicine Immunology and Allergy Animals Fibroblast Receptor Cytotoxicity Lymph node Cells Cultured Rats Inbred Strains Receptors Antigen T-Cell gamma-delta Original Articles In vitro Recombinant Proteins Rats medicine.anatomical_structure Cytomegalovirus Infections Cytokines Interleukin-2 Lymph Nodes medicine.drug |
| Zdroj: | Immunology. 108(2) |
| ISSN: | 0019-2805 |
| Popis: | Gammadelta T cells are unique, and their localization at sites of infection is considered critical in immune defence. We demonstrate the accumulation of gammadelta T cells in rat regional popliteal lymph nodes (PLNi) starting 2 days after inoculation of cytomegalovirus (CMV) into the footpad. Early-appearance PLNi gammadelta T cells significantly inhibited plaque development and the spread of CMV infection. These gammadelta T cells were negative for CD4 and CD8beta receptors, proliferated in response to interleukin-2 (IL-2) and contained high levels of interferon-gamma (IFN-gamma), the appearance of which correlated with the curing of fibroblasts from virus infection. The addition of anti-IFN-gamma abolished the ability of fibroblast monolayers to be cured from CMV infection. In contrast, this protection was not abolished by the addition of anti-rat IL-2 or anti-rat TNF-alpha, or by the depletion of NKR-P1-bearing cells within gammadelta T cells. In addition, the present study shows that while gammadelta T cells derived from naive and CMV-infected rats are able to kill both YAC-1 targets and CMV-infected syngeneic fibroblasts in vitro, only the latter are able to clear CMV-infected fibroblast monolayers. Finally, our data suggest that the expression of NKR-P1 by gammadelta T cells is critical for cytotoxicity, but its contribution to the curing from CMV infection was limited. |
| Databáze: | OpenAIRE |
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