African Swine Fever Virus Structural Protein p17 Inhibits Cell Proliferation through ER Stress—ROS Mediated Cell Cycle Arrest
| Autor: | Wanglong Zheng, Hui Wang, Sen Jiang, Nanhua Chen, Jia Luo, Da Ao, Jiajia Zhang, Xueliang Liu, Yulin Xu, François Meurens, Nengwen Xia, Qi Shao, Jianzhong Zhu |
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| Přispěvatelé: | Yangzhou University, Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The work was partly supported by the National Key Research and Development Program of China (2017YFD0502301), Jiangsu provincial key R & D plan (BE2020398), National Natural Science Foundation of China (31872450, 31672523), and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Gene Expression Regulation
Viral 0301 basic medicine Cell cycle checkpoint Swine viruses [SDV]Life Sciences [q-bio] lcsh:QR1-502 Apoptosis Cell cycle Endoplasmic Reticulum medicine.disease_cause African swine fever virus Article lcsh:Microbiology Cell Line 03 medical and health sciences p17 protein immune system diseases Virology medicine Animals Humans African Swine Fever Cell Proliferation Viral Structural Proteins L-Lactate Dehydrogenase 030102 biochemistry & molecular biology biology Chemistry Cell growth Endoplasmic reticulum virus diseases ROS Cell Cycle Checkpoints Endoplasmic Reticulum Stress biology.organism_classification Cell biology 030104 developmental biology Infectious Diseases Host-Pathogen Interactions Unfolded protein response Reactive Oxygen Species ER stress Oxidative stress |
| Zdroj: | Viruses Viruses, MDPI, 2021, 13 (1), pp.21. ⟨10.3390/v13010021⟩ Volume 13 Issue 1 Viruses, Vol 13, Iss 21, p 21 (2021) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v13010021⟩ |
| Popis: | International audience; African swine fever virus (ASFV) is a highly pathogenic large DNA virus that causes African swine fever (ASF) in domestic pigs and wild boars. The p17 protein, encoded by the D117L gene, is a major transmembrane protein of the capsid and the inner lipid envelope. The aim of this study was to investigate the effects of p17 on cell proliferation and the underlying mechanisms of action. The effects of p17 on cell proliferation, cell cycle, apoptosis, oxidative stress, and endoplasmic reticulum (ER) stress have been examined in 293T, PK15, and PAM cells, respectively. The results showed that p17 reduced cell proliferation by causing cell cycle arrest at G2/M phase. Further, p17-induced oxidative stress and increased the level of intracellular reactive oxygen species (ROS). Decreasing the level of ROS partially reversed the cell cycle arrest and prevented the decrease of cell proliferation induced by p17 protein. In addition, p17-induced ER stress, and alleviating ER stress decreased the production of ROS and prevented the decrease of cell proliferation induced by p17. Taken together, this study suggests that p17 can inhibit cell proliferation through ER stress and ROS-mediated cell cycle arrest, which might implicate the involvement of p17 in ASF pathogenesis. |
| Databáze: | OpenAIRE |
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