Applying a novel 3D hydrogel cell culture to investigate activation of microglia due to rotational kinematics associated with mild traumatic brain injury
| Autor: | Lowell McAllister, Christopher R. Dennison, Matthew A. Churchward, Kathryn G. Todd, Samantha R. MacGillivray, Savannah M. Weber, Christopher Tsui |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Traumatic brain injury
medicine.medical_treatment Cell Cell Culture Techniques Biomedical Engineering 02 engineering and technology Kinematics Nitric oxide Biomaterials 03 medical and health sciences chemistry.chemical_compound 3D cell culture 0302 clinical medicine medicine Humans Brain Concussion Microglia Hydrogels 030206 dentistry 021001 nanoscience & nanotechnology medicine.disease Biomechanical Phenomena medicine.anatomical_structure Cytokine chemistry Mechanics of Materials Cell culture 0210 nano-technology Neuroscience |
| Zdroj: | Journal of the Mechanical Behavior of Biomedical Materials. 114:104176 |
| ISSN: | 1751-6161 |
| DOI: | 10.1016/j.jmbbm.2020.104176 |
| Popis: | Many investigations on mild traumatic brain injury (mTBI) aim to further understand how cells in the brain react to the mechanical forces associated with the injury. While it is known that rapid head rotation is a mechanism contributing to mTBI, establishing definitive thresholds for head rotation has proved challenging. One way to advance determining mechanisms and thresholds for injury is through in vitro models. Here, an apparatus has been designed that is capable of delivering rotational forces to three-dimensional (3D) hydrogel cell cultures. Using an in vitro model, we test the hypothesis that rotational kinematics can activate microglia suspended in a 3-dimensional mixed glia environment (absent neurons). The impact apparatus was able to deliver peak angular velocities of approximately 45 rad/s, a magnitude for angular velocity that in select literature is associated with diffuse brain injury. However, no measurable glial cell reactivity was observed in response to the rotational kinematics through any of the chosen metrics (nitric oxide, pro-inflammatory cytokine release and proportion of amoeboid activated microglia). The results generated from this study suggest that rotation of the glia alone did not cause activation - in future work we will investigate the effect of neuronal contributions in activating glia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect