Genetic structure of Plasmodium falciparum populations across the Honduras-Nicaragua border
| Autor: | Nerea Larranaga, Alberto Montoya, Aida Soto, Gustavo Fontecha, Rosa E Mejía, José I. Hormaza |
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| Jazyk: | angličtina |
| Předmět: |
Linkage disequilibrium
Endemic Diseases Population Plasmodium falciparum Zoology Nicaragua Population structure Genetic diversity Genetic variation parasitic diseases medicine Cluster Analysis Humans Malaria Falciparum education Microsatellites Phylogeny education.field_of_study Molecular Epidemiology biology Molecular epidemiology Ecology Research Genetic Variation DNA Protozoan biology.organism_classification medicine.disease Infectious Diseases Honduras Genetic structure Parasitology Malaria Microsatellite Repeats |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Malaria Journal |
| ISSN: | 1475-2875 |
| DOI: | 10.1186/1475-2875-12-354 |
| Popis: | Background The Caribbean coast of Central America remains an area of malaria transmission caused by Plasmodium falciparum despite the fact that morbidity has been reduced in recent years. Parasite populations in that region show interesting characteristics such as chloroquine susceptibility and low mortality rates. Genetic structure and diversity of P. falciparum populations in the Honduras-Nicaragua border were analysed in this study. Methods Seven neutral microsatellite loci were analysed in 110 P. falciparum isolates from endemic areas of Honduras (n = 77) and Nicaragua (n = 33), mostly from the border region called the Moskitia. Several analyses concerning the genetic diversity, linkage disequilibrium, population structure, molecular variance, and haplotype clustering were conducted. Results There was a low level of genetic diversity in P. falciparum populations from Honduras and Nicaragua. Expected heterozigosity (H e ) results were similarly low for both populations. A moderate differentiation was revealed by the FST index between both populations, and two putative clusters were defined through a structure analysis. The main cluster grouped most of samples from Honduras and Nicaragua, while the second cluster was smaller and included all the samples from the Siuna community in Nicaragua. This result could partially explain the stronger linkage disequilibrium (LD) in the parasite population from that country. These findings are congruent with the decreasing rates of malaria endemicity in Central America. We would like to thank Dr Udhayakumar Venkatachalam and his research team at Malaria Branch, CDC, for technical assistance and providing amplification protocols and DNA of reference clones. DNA from reference clones was also provided by Irina Jovel from Karolinska Institute, Sweden. The financial support for this work was provided by CSIC (I-COOP0071). Complementary funds were provided by Direccion de Investigacion Cientifica – UNAH. |
| Databáze: | OpenAIRE |
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