Water extract of ginseng and astragalus regulates macrophage polarization and synergistically enhances DDP's anticancer effect
| Autor: | Yingna Chen, Huijuan Luo, Weiping Chen, Yu-Cui Jiang, Lei Bi, Feiyan Chen, Yunshan Wang, Guangwei Wei |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Lung Neoplasms
medicine.medical_treatment Macrophage polarization Panax Antineoplastic Agents Pharmacology 03 medical and health sciences Ginseng Carcinoma Lewis Lung 0302 clinical medicine In vivo Cell Line Tumor Drug Discovery medicine Tumor Microenvironment Animals Humans 030304 developmental biology Cisplatin A549 cell 0303 health sciences Tumor microenvironment Chemistry Plant Extracts Macrophages Cell Polarity Water Drug Synergism Astragalus Plant Mice Inbred C57BL Cytokine 030220 oncology & carcinogenesis Cancer cell Solvents Cytokines Female medicine.drug |
| Zdroj: | Journal of ethnopharmacology. 232 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance In traditional Chinese medicine, supplementing Qi and strengthening body resistance are an important principle of anticancer treatment. Panax ginseng C.A.Mey. (ginseng) and Astragalus membranaceus Bunge (astragalus) are the representative herbs for this therapeutic principle. Aim of the study This study aims to explore the effect of the water extract of ginseng and astragalus (WEGA) on regulating macrophage polarization and mediating anticancer in the tumor microenvironment. Materials and methods A549 cells were cultured in tumor-associated macrophage (TAM) supernatant with various concentrations of WEGA (0, 5, 10, 20 mg/mL). A549 cell proliferation was determined through methyl thiazole tetrazolium (MTT) assay and real-time cell analysis (RTCA), respectively. In vivo experiments were performed with a Lewis lung cancer (LLC) xenograft mouse model. Forty-eight mice were divided into six groups and treated with saline, WEGA, or cis-diamine dichloro platinum (DDP) with dosage of WEGA (0, 30, 60, 120 mg/kg body weight/day). The different groups were administered with drugs via oral or intraperitoneal injection once a day for 21 consecutive days. Tumor inhibition rate, spleen index, thymus index, cytokine, protein, and mRNA expression levels were detected in mice. Results In a co-culture system, WEGA remarkably inhibited A549 cell proliferation, promoted the expression of M1 macrophage markers and inhibited M2 TAMs markers. Therefore, WEGA affected the biological behavior of cancer cells by regulating the expression of some markers relevant to macrophage polarization. In addition, the group of WEGA and DDP chemotherapy effectively inhibited the transplanted tumor growth in mice and improved weight loss and immunosuppressive with the cisplatin inducing. Conclusions This study provides mechanistic insights into the anticancer effect of WEGA through the regulation of macrophage polarization and highlights that WEGA could be a novel option for integrative cancer therapies. |
| Databáze: | OpenAIRE |
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