Polymeric Sequence Probe for Single DNA Detection
| Autor: | Yong Chen, Suxian Huang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Chemistry
Oligonucleotide Polynucleotides DNA Single-Stranded Sequence (biology) Mycobacterium tuberculosis Fluorescence Molecular biology eye diseases Analytical Chemistry chemistry.chemical_compound Tandem repeat Polynucleotide Biophysics Fluorescence microscope Molecule DNA Probes Cells Cultured DNA |
| Zdroj: | Analytical Chemistry. 83:7250-7254 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac201837p |
| Popis: | A polynucleotide probe, polymeric sequence probe (PSP), was developed for single molecular detections. PSP is a single-stranded DNA molecule with ~2000 tandem repeat target-binding sequences and label-binding sequences. A single PSP can bind to multiple fluorescent complementary oligos to generate a strong fluorescence signal. Single target molecules bound to PSPs can be clearly visualized by a conventional fluorescence microscope. An ultrasensitive PSP-based assay for Mycobacterium tuberculosis was demonstrated. |
| Databáze: | OpenAIRE |
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