Functional conservation of EXA1 among diverse plant species for the infection by a family of plant viruses
Autor: | Osamu Netsu, Akira Yusa, Masayoshi Hashimoto, Shigetou Namba, Kai Tokumaru, Naoi Hosoe, Yasuyuki Yamaji, Tetsuya Yoshida, Takuya Keima, Yutaro Neriya, Kensaku Maejima, Yuji Fujimoto |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
viruses lcsh:Medicine Nicotiana benthamiana Article 03 medical and health sciences 0302 clinical medicine Solanum lycopersicum Gene Expression Regulation Plant Plant virus Tobacco Gene silencing Arabidopsis thaliana lcsh:Science Gene Plant Diseases Plant Proteins Genetics Multidisciplinary biology Host (biology) lcsh:R fungi food and beverages biology.organism_classification Potexvirus 030104 developmental biology Host-Pathogen Interactions lcsh:Q 030217 neurology & neurosurgery Solanaceae |
Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
ISSN: | 2045-2322 |
Popis: | Since the propagation of plant viruses depends on various host susceptibility factors, deficiency in them can prevent viral infection in cultivated and model plants. Recently, we identified the susceptibility factor Essential for poteXvirus Accumulation 1 (EXA1) in Arabidopsis thaliana, and revealed that EXA1-mediated resistance was effective against three potexviruses. Although EXA1 homolog genes are found in tomato and rice, little is known about which viruses depend on EXA1 for their infection capability and whether the function of EXA1 homologs in viral infection is conserved across multiple plant species, including crops. To address these questions, we generated knockdown mutants using virus-induced gene silencing in two Solanaceae species, Nicotiana benthamiana and tomato. In N. benthamiana, silencing of an EXA1 homolog significantly compromised the accumulation of potexviruses and a lolavirus, a close relative of potexviruses, whereas transient expression of EXA1 homologs from tomato and rice complemented viral infection. EXA1 dependency for potexviral infection was also conserved in tomato. These results indicate that EXA1 is necessary for effective accumulation of potexviruses and a lolavirus, and that the function of EXA1 in viral infection is conserved among diverse plant species. |
Databáze: | OpenAIRE |
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