Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters▿
| Autor: | Achim Heck, Jochen Büchs, Franco Circolone, Robert Huber, Anne-Kathrin Hillmer, Karl-Erich Jaeger, Thomas Drepper |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Time Factors
Flavin Mononucleotide Green Fluorescent Proteins Flavin mononucleotide Flavin group Biology medicine.disease_cause Applied Microbiology and Biotechnology Green fluorescent protein chemistry.chemical_compound Bimolecular fluorescence complementation In vivo Genes Reporter medicine Methods Escherichia coli Ecology Escherichia coli Proteins Fluorescence Oxygen chemistry Biochemistry Quantitative analysis (chemistry) Food Science Biotechnology |
| Popis: | Fluorescent proteins of the green fluorescent protein (GFP) family are commonly used as reporter proteins for quantitative analysis of complex biological processes in living microorganisms. Here we demonstrate that the fluorescence signal intensity of GFP-like proteins is affected under oxygen limitation and therefore does not reflect the amount of reporter protein in Escherichia coli batch cultures. Instead, flavin mononucleotide (FMN)-binding fluorescent proteins (FbFPs) are suitable for quantitative real-time in vivo assays under these conditions. |
| Databáze: | OpenAIRE |
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