Genome-wide identification of OTP gene as a novel methylation marker of breast cancer
| Autor: | Myung Soon Kim, Kwang Sun Seo, Jeung-Hoon Lee, Eilsung Chang, Sungwhan An, Benjamin D. Hoehn, Youngho Moon, TaeJeong Oh, Jinsun Lee |
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| Rok vydání: | 2011 |
| Předmět: |
Oncology
Adult Cancer Research medicine.medical_specialty LIM-Homeodomain Proteins Breast Neoplasms Nerve Tissue Proteins Biology medicine.disease_cause Breast cancer Internal medicine Cell Line Tumor medicine Biomarkers Tumor Humans WT1 Proteins Aged Homeodomain Proteins Gene Expression Profiling Cancer General Medicine Methylation DNA Methylation Middle Aged medicine.disease Primary tumor Gene expression profiling CpG site DNA methylation Cancer research Female Carcinogenesis Genome-Wide Association Study Transcription Factors |
| Zdroj: | Oncology reports. 27(5) |
| ISSN: | 1791-2431 |
| Popis: | Aberrant DNA methylation occurs early and frequently in tumorigenesis. Identification of DNA methylation biomarkers is a field that provides potential for improving the clinical process of breast cancer diagnosis. We utilized a genome-wide technique, methylated DNA isolation assay (MeDIA), in combination with high-resolution CpG microarray analysis to identify hypermethylated genes in breast cancer. Among differentially methylated genes between tumor and adjacent normal tissues, 3 candidate genes (LHX2, WT1 and OTP) were finally selected through a step-wise filtering process and examined for methylation status in normal tissues, primary tumor, and paired adjacent normal-appearing tissues from 39 breast cancer patients. Based on the calculated cut-off values, all genes showed significantly higher frequencies of aberrant hypermethylation in primary tumors (43.6% for LHX2, 89.7% for WT1 and 100% for OTP, p |
| Databáze: | OpenAIRE |
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