Expression of Ceruloplasmin in the Peripheral Blood of Patients With Drug‐Resistant Epilepsy

Autor: Hai‐Yan Chen, Yao‐Xin Pan, Xue‐Bin Li, Yan‐Fang Yun, Gui‐Xin Yang, Yong‐Ming Jiang, Dong Lu, Jian‐Min Huang
Rok vydání: 2023
Předmět:
Zdroj: The Journal of Clinical Pharmacology. 63:466-472
ISSN: 1552-4604
0091-2700
DOI: 10.1002/jcph.2183
Popis: This study was performed to detect the expression of ceruloplasmin in the peripheral blood of patients with drug-resistant epilepsy and explore the mechanisms of iron metabolism disorder in drug-resistant epilepsy. The expression levels of ceruloplasmin mRNA and ceruloplasmin protein in the peripheral blood of the three groups were detected using real-time fluorescence-based quantitative polymerase chain reaction and Western blot. The differences in the expression of ceruloplasmin mRNA of different seizure frequencies and types, electroencephalogram (EEG) abnormal discharges, and different medication methods were analyzed and compared. The relative expression of ceruloplasmin mRNA and ceruloplasmin protein in the drug-resistant epilepsy group was significantly higher than that in the drug responsive group and higher in the drug responsive group compared with the normal group. The relative expression of ceruloplasmin mRNA in patients with epilepsy using different medication methods was statistically significant. Patients who received a combination of two or three drugs exhibited a higher expression than those treated with a single-drug treatment, whereas those who received a combination of three drugs had a higher expression than those with two drugs. The increased expression of ceruloplasmin in the peripheral blood of patients with drug-resistant epilepsy was closely related to the different medication methods, but no obvious correlation with epileptic seizure frequencies or types and abnormal EEG discharges was identified. The increased expression of ceruloplasmin enhanced iron oxidative damage and may be the potential mechanism of drug-resistant epilepsy and may be one of the drug resistance indicators for combination drugs when treating drug-resistant epilepsy. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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