New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium)
| Autor: | Jia-Hui Wang, Da-Gang Hu, Fangfang Ma, Cheng-Shu Zheng, Cui-Hui Sun, Peng Zhang, Kai-Di Gu, Xinyi Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
Chrysanthemum
Nitrogen assimilation Citric Acid Cycle MADS Domain Proteins Plant Science Genes Plant Models Biological Plant Roots Gene Expression Regulation Plant lcsh:Botany CmANR1 Metabolomics Photosynthesis Transcription factor MADS-box Principal Component Analysis Nitrates biology Gene Expression Profiling Chrysanthemum morifolium Shoot biology.organism_classification lcsh:QK1-989 Root development Cell biology Citric acid cycle Metabolic pathway Transcriptome Transcription Factor Gene Glycolysis Plant Shoots Signal Transduction Research Article |
| Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 21, Iss 1, Pp 1-16 (2021) |
| ISSN: | 1471-2229 |
| Popis: | Background MADS-box transcription factors (TFs) are the key regulators of multiple developmental processes in plants; among them, a chrysanthemum MADS-box TF CmANR1 has been isolated and described as functioning in root development in response to high nitrate concentration signals. However, how CmANR1 affects root and shoot development remains unclear. Results We report that CmANR1 plays a positive role in root system development in chrysanthemum throughout the developmental stages of in vitro tissue cultures. Metabolomics combined with transcriptomics assays show that CmANR1 promotes robust root system development by facilitating nitrate assimilation, and influencing the metabolic pathways of amino acid, glycolysis, and the tricarboxylic acid cycle (TCA) cycle. Also, we found that the expression levels of TFs associated with the nitrate signaling pathways, such as AGL8, AGL21, and LBD29, are significantly up-regulated in CmANR1-transgenic plants relative to the wild-type (WT) control; by contrast, the expression levels of RHD3-LIKE, LBD37, and GATA23 were significantly down-regulated. These results suggest that these nitrate signaling associated TFs are involved in CmANR1-modulated control of root development. In addition, CmANR1 also acts as a positive regulator to control shoot growth and development. Conclusions These findings provide potential mechanisms of MADS-box TF CmANR1 modulation of root and shoot development, which occurs by regulating a series of nitrate signaling associated TFs, and influencing the metabolic pathways of amino acid and glycolysis, as well as TCA cycle and nitrate assimilation. |
| Databáze: | OpenAIRE |
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