Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
| Autor: | Joaquín J. Salas, Raquel Martins-Noguerol, Brigitte Thomasset, Mónica Venegas-Calerón, Antonio J. Moreno-Pérez, Enrique Martínez-Force, Sébastien Acket, M. Adrián Troncoso-Ponce, Rafael Garcés |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Universidad de Sevilla. Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Génie Enzymatique et Cellulaire (GEC), Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Instituto de la Grasa, Consejo Superior de Investigaciones Científicas [Madrid] (CSIC) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
sunflower mitochondrial lipoylation lipoyl synthase Plant Science Oxidative phosphorylation Mitochondrion 01 natural sciences Cofactor SB1-1110 03 medical and health sciences chemistry.chemical_compound Lipoyl synthase Octanoyltransferase [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Fatty acid synthesis 030304 developmental biology chemistry.chemical_classification 0303 health sciences Lipoic acid biology Chemistry Fatty acid Plant culture [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics Pyruvate dehydrogenase complex lipoic acid Sunflower Enzyme Biochemistry octanoyltransferase biology.protein 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 12 (2021) Digital.CSIC. Repositorio Institucional del CSIC instname idUS. Depósito de Investigación de la Universidad de Sevilla Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2021, 12, pp.781917. ⟨10.3389/fpls.2021.781917⟩ |
| ISSN: | 1664-462X |
| Popis: | 18 Páginas.-- 12 Figuras.-- 2 Tablas Lipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of the octanoyltransferase (LIP2, EC 2.3.1.181) and lipoyl synthase (LIP1, EC 2.8.1.8) enzymes. In plants, pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase or glycine decarboxylase are essential complexes that need to be lipoylated. These lipoylated enzymes and complexes are located in the mitochondria, while PDH is also present in plastids where it provides acetyl-CoA for de novo fatty acid biosynthesis. As such, lipoylation of PDH could regulate fatty acid synthesis in both these organelles. In the present work, the sunflower LIP1 and LIP2 genes (HaLIP1m and HaLIP2m) were isolated sequenced, cloned, and characterized, evaluating their putative mitochondrial location. The expression of these genes was studied in different tissues and protein docking was modeled. The genes were also expressed in Escherichia coli and Arabidopsis thaliana, where their impact on fatty acid and glycerolipid composition was assessed. Lipidomic studies in Arabidopsis revealed lipid remodeling in lines overexpressing these enzymes and the involvement of both sunflower proteins in the phenotypes observed is discussed in the light of the results obtained. This work was financed by the PID2020-113134RB-I00/AEI/10.13039/501100011033 project granted by the Spanish State Research Agency within the State Programs for Research and Innovation Oriented to the Challenges of Society. |
| Databáze: | OpenAIRE |
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