| Autor: |
Dar Heinze, Seonmi Park, Andrew McCracken, Mona Haratianfar, Jonathan Lindstrom, Carlos Villacorta-Martin, Aditya Mithal, Feiya Wang, Meng Wei Yang, George Murphy, Gustavo Mostoslavsky |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Stem cell reports. 17(12) |
| ISSN: |
2213-6711 |
| Popis: |
A robust method of producing mature T cells from iPSCs is needed to realize their therapeutic potential. NOTCH1 is known to be required for the production of hematopoietic progenitor cells with T cell potential in vivo. Here we identify a critical window during mesodermal differentiation when Notch activation robustly improves access to definitive hematopoietic progenitors with T/NK cell lineage potential. Low-density progenitors on either OP9-hDLL4 feeder cells or hDLL4-coated plates favored T cell maturation into TCRab |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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