Genotipos deNeisseria meningitidisaislados de pacientes con enfermedad meningocócica en Paraguay, 1996-2015
| Autor: | Gustavo Chamorro, Grupo del laboratorio Vimene Paraguay, Minako Nagai, Aníbal Kawabata, Liliana Rojas, María Eugenia León |
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| Rok vydání: | 2019 |
| Předmět: |
lcsh:Arctic medicine. Tropical medicine
Paraguai lcsh:RC955-962 polymerase chain reaction enfermedad meningocócica lcsh:Medicine Meningococcal Infections multilocus sequence typing tipificación de secuencias multilocus tipagem de sequências multilocus Neisseria meningitidis Biology Meningococcal disease medicine.disease_cause medicine reaçâo em cadeia da polimerase Investigación Original doença meningocócica meningococcal disease lcsh:Public aspects of medicine lcsh:R Public Health Environmental and Occupational Health lcsh:RA1-1270 medicine.disease Molecular biology reacción en cadena de la polimerasa Paraguay |
| Zdroj: | Revista Panamericana de Salud Pública, Vol 43, Pp 1-8 (2019) Revista Panamericana de Salud Pública |
| ISSN: | 1680-5348 1020-4989 |
| Popis: | Describe the phenotypical and genotypical characteristics ofConventional microbiological methods and molecular techniques were used to study 114 isolates of N. meningitidis and 12 clinical samples without isolation (confirmed by polymerase chain reaction), provided by various sentinel centers and collaborating centers in Paraguay.The most affected age group was children under 1 year (19.0%), followed by 1-5-year-olds (17.5%). The highest percentage of strains was isolated in meningitis cases (81.7%) and serogroup B was found in 60.3% of cases. The most frequent phenotypes were B:4:p1.14 (16.0%), B:15:p1.5, C:nt:nst, and W:nt:p1.2 (10.5%), respectively. The prevalent clonal complexes were: ST-11/ET37 complex, 29.6% (8/27), predominantly serogroup W (6/8); ST-35 complex, 18.5% (5/27), in serogroup B (4/4); and ST-32/ET5 complex, 14.8% (4/16), in serogroup B (5/5).Meningococcal meningitis is relatively uncommon in Paraguay. Distribution analysis showed that serogroup B is the most common and that the number of cases of meningococcal disease caused by serogroups C and W increased in the last two years. The identified clonal complexes were correlated with those found in the Southern Cone region. Due to the high virulence ofDescrever as características fenotípicas e genotípicas de cepas de Neisseria meningitidis isoladas de casos de doença meningocócica no Paraguai entre 1996 e 2015.Foram estudados por métodos microbiológicos convencionais e técnicas moleculares 114 isolados deA faixa etária mais afetada foi a de crianças menores de 1 ano (19,0%) e crianças de 1 a 5 anos (17,5%). Uma maior porcentagem de cepas foi isolada de casos de meningite (81,7%) e o sorogrupo B foi identificado em 60,3% dos casos. Os fenótipos mais comuns foram B:4:P1.14 (16,0%), B:15:P1.5, C:NT:NST e W:NT:P1.2 (10,5%), respectivamente. Os complexos clonais mais prevalentes foram o complexo ST-11/ET37 (29,6%, 8/27) com predomínio no sorogrupo W (6/8), complexo ST-35 (18,5%, 5/27) no sorogrupo B (4/4) e complexo ST-32/ET5 (14,8%, 4/16) no sorogrupo B (5/5).A doença meningocócica é relativamente pouco comum no Paraguai. A análise da distribuição dos sorogrupos demonstrou que o sorogrupo B é o mais prevalente e, nos últimos dois anos, ouve um aumento nos casos de doença meningocócica pelos sorogrupos C e W. Os complexos clonais encontrados se correlacionam com os achados na região do Cone Sul. Devido à alta virulência da |
| Databáze: | OpenAIRE |
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