Effect of combined locally delivered growth factors and systemic sildenafil citrate on microrecanalization in biodegradable conduit for vas deferens reconstruction
| Autor: | Carlos Atico Ariza, Surya K. Mallapragada, Moshe Wald, Yogita Krishnamachari, Thomas S. Griffith, Barry R. De Young, Aliasger K. Salem, Henry M. Rosevear |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Sildenafil Urology Bilateral vasectomy medicine.medical_treatment Rat model Placebo Piperazines Sildenafil Citrate chemistry.chemical_compound Vas Deferens Transforming Growth Factor beta Absorbable Implants Medicine Animals Sulfones business.industry Growth factor Microcirculation Vasovasostomy Proto-Oncogene Proteins c-sis Rats Vas deferens surgery chemistry Purines Anesthesia Vas deferens reconstruction cardiovascular system Nanoparticles business |
| Zdroj: | Urology. 79(4) |
| ISSN: | 1527-9995 |
| Popis: | To investigate the effect of the combination of locally delivered growth factors and oral sildenafil citrate on cross-conduit microrecanalization.A total of 42 rats were divided into 7 groups. Of the 42 rats, 6 underwent bilateral vasectomy and bilateral end-to-end vasovasostomy and 12 underwent bilateral vasectomy. Of the latter 12, 6 received sildenafil citrate orally (10 mg/kg/d) for 24 weeks and 6 received placebo. A total of 24 rats underwent bilateral vasectomy and bilateral reconstruction with implantation of a 5-mm biodegradable conduit that bridged the 2 vasal ends. Of the 24 rats with conduits, 12 also had 250 pg of transforming growth factor-β and 12.5 pg of platelet-derived growth factor-β sustained release nanoparticles placed in immediate proximity to the conduit. The remaining 12 rats with conduits (6 without growth factors and 6 with growth factors) also received sildenafil citrate orally (10 mg/kg/d) for 24 weeks; the others received placebo. The reconstructed segments were harvested for histologic examination at 24 weeks.Five of 6 primary vasovasostomy and no vasectomy-only rats sired litters. Significantly more microcanals per conduit were observed in rats receiving sildenafil citrate: without growth factors, 3.9 vs. 0 canals/conduit (P0.001); with growth factors, 5.5 vs. 0.25 canals/conduit (P0.001). The rats receiving sildenafil citrate with growth factors showed a trend toward more microcanals per conduit than the rats receiving sildenafil citrate without growth factors (5.5 vs 3.9; P = .10). Rats receiving growth factors but no sildenafil citrate did not produce more canals than the rats receiving neither growth factor nor sildenafil citrate (0.25 vs 0; P = NS).Orally administered sildenafil citrate enhances formation of microcanalization after postvasectomy reconstruction using a biodegradable conduit in a rat model. Locally delivered growth factors appear to increase the number of microcanals. |
| Databáze: | OpenAIRE |
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