Immunosuppressive properties of Pluchea lanceolata leaves
| Autor: | Naresh Kumar Satti, Sarang Bani, DP Bhagwat, Kiranjeet Kour, Kharya, Prashant Singh Chauhan, K. A. Suri, Anpurna Kaul |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Pharmacology
Hemagglutination cell-mediated immune response Phagocytosis medicine.medical_treatment CD 4 T-helper cells Antibody titer Immunosuppression Biology In vitro Immune system Antigen In vivo Pluchea lanceolata Immunology medicine CD 8 T-cytotoxic cells Pharmacology (medical) Humoral antibody titer Research Article |
| Zdroj: | Indian Journal of Pharmacology |
| ISSN: | 1998-3751 0253-7613 |
| Popis: | Objective: To investigate the immunosuppressive potential of Pluchea lanceolata 50% ethanolic extract (PL) and its bioactive chloroform fraction (PLC). Materials and Methods: Preliminary screening of the Pluchea lanceolata 50% ethanolic extract (PL) was carried out with basic models of immunomodulation, such as, the humoral antibody response (hemagglutination antibody titers), cell-mediated immune response (delayed-type hypersensitivity), skin allograft rejection test, in vitro (C. albicans method), and in vivo phagocytosis (carbon clearance test). The extract was then fractionated with chloroform, n-butanol, and water to receive the respective fractions by partitioning. These fractions were employed for flow cytometry to study the T-cell specific immunosuppressive potential of these fractions. Results: Oral administration of PL at doses of 50 to 800 mg/kg in mice, with sheep red blood cells (SRBC) as an antigen, inhibited both humoral and cell-mediated immune responses, as evidenced by the production of the circulating antibody titer and delayed-type hypersensitiviy reaction results, respectively, and the immune suppression was statistically significant (P < 0.01) in Balb/C mice. PL also decreased the process of phagocytosis both in vitro (31.23%) and ex vivo (32.81%) and delayed the graft rejection time (30.76%). To study the T-cell-specific activities, chloroform, n-butanol, and water fractions from P. lanceolata were tested for T-cell specific immunosuppressive evaluation, wherein only the chloroform fraction (PLC) showed significant (P < 0.01) suppression of CD8+ / CD4+ T-cell surface markers and intracellular Th1 (IL-2 and IFN-Y) cytokines at 25 – 200 mg/kg p.o. doses. PLC, however, did not show significant suppression of the Th2 (IL-4) cytokine. Conclusion: The findings from the present investigation reveal that P. lanceolata causes immunosuppression by inhibiting Th1 cytokines. |
| Databáze: | OpenAIRE |
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