Puromycin‐sensitive aminopeptidase is required for C2C12 myoblast proliferation and differentiation
| Autor: | Hiroaki Takada, Takahiro Kubota, Ryoichi Nagatomi, Yasuo Kitajima, Kazutaka Murayama, Aki Nunomiya, Naoki Suzuki, Shion Osana |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Myoblast proliferation Physiology Clinical Biochemistry Protein degradation myogenic differentiation Muscle Development Aminopeptidase Aminopeptidases Cell Line Puromycin-Sensitive Aminopeptidase Myoblasts 03 medical and health sciences Myoblast fusion puromycin‐sensitive aminopeptidase Mice 0302 clinical medicine myoblast fusion Animals Research Articles Cell Proliferation Myogenesis Chemistry Cell Differentiation Cell Biology musculoskeletal system Cell biology cell polarity 030104 developmental biology proteasome 030220 oncology & carcinogenesis C2C12 tissues Intracellular Research Article |
| Zdroj: | Journal of Cellular Physiology |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | The ubiquitin‐proteasome system is a major protein degradation pathway in the cell. Proteasomes produce several peptides that are rapidly degraded to free amino acids by intracellular aminopeptidases. Our previous studies reported that proteolysis via proteasomes and aminopeptidases is required for myoblast proliferation and differentiation. However, the role of intracellular aminopeptidases in myoblast proliferation and differentiation had not been clarified. In this study, we investigated the effects of puromycin‐sensitive aminopeptidase (PSA) on C2C12 myoblast proliferation and differentiation by knocking down PSA. Aminopeptidase enzymatic activity was reduced in PSA‐knockdown myoblasts. Knockdown of PSA induced impaired cell cycle progression in C2C12 myoblasts and accumulation of cells at the G2/M phase. Additionally, after the induction of myogenic differentiation in PSA‐knockdown myoblasts, multinucleated circular‐shaped myotubes with impaired cell polarity were frequently identified. Cell division cycle 42 (CDC42) knockdown in myoblasts resulted in a loss of cell polarity and the formation of multinucleated circular‐shaped myotubes, which were similar to PSA‐knockdown myoblasts. These data suggest that PSA is required for the proliferation of myoblasts in the growth phase and for the determination of cell polarity and elongation of myotubes in the differentiation phase. Knockdown of PSA induced impaired cell cycle progression in C2C12 myoblasts and accumulation of cells at the G2/M phase. Additionally, after the induction of myogenic differentiation in PSA‐knockdown myoblasts, multinucleated circular‐shaped myotubes with impaired cell polarity were frequently identified. These data suggest that PSA is required for the proliferation of myoblasts in the growth phase and for the determination of cell polarity and elongation of myotubes in the differentiation phase. |
| Databáze: | OpenAIRE |
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