Purification and characterization of anticoagulant protein from the tabanus, Tabanus bivittatus
| Autor: | Mi Young Ahn, Bum Soo Hahn, Song Ji Wu, Pyeong Jae Lee, Yeong Shik Kim |
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| Rok vydání: | 2006 |
| Předmět: |
Cations
Divalent Protein Conformation Thrombin time Drug Discovery medicine Animals education Blood Coagulation Gel electrophoresis education.field_of_study Chromatography medicine.diagnostic_test Tabanus biology Isoelectric focusing Chemistry Diptera Organic Chemistry Temperature Thrombin Anticoagulants Hydrogen-Ion Concentration biology.organism_classification Molecular Weight Zinc Isoelectric point Clotting time Biochemistry Chromogenic Compounds Molecular Medicine Coagulation screen Insect Proteins Partial Thromboplastin Time Copper Partial thromboplastin time |
| Zdroj: | Archives of pharmacal research. 29(5) |
| ISSN: | 0253-6269 |
| Popis: | Tabanus anticoagulant protein (TAP) was isolated from the whole body of the tabanus, Tabanus bivittatus, using three purification steps (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and ion exchange chromatography on DEAE Sephadex gel). The purified TAP, with a molecular weight of 65 kDa, was assessed to be homogeneous by SDS-polyacrylamide gel electrophoresis, and an isoelectric point of 7.9 was determined by isoelectric focusing. The internal amino acid sequence of the purified protein was composed of Ser-Leu-Asn-Asn-Gln-Phe-Ala-Ser-Phe-Ile-Asp-Lys-Val-Arg. The protein was activated by Cu2+ and Zn2+, and the optimal conditions were found to be at pH 3-6 and 40-70 degrees C. Standard coagulation screen assays were used to determine thrombin time and activated partial thromboplastin time. Chromogenic substrate assays were performed for thrombin and factor Xa activity. TAP considerably prolonged human plasma clotting time, especially activated partial thromboplastin time in a dose-dependent manner; it showed potent and specific antithrombin activity in the chromogenic substrate assay. Specific anti-factor Xa activity in TAP was not detected. Overall, this result suggested that TAP has significant anticoagulant activity on blood coagulation system. |
| Databáze: | OpenAIRE |
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