Serologic Responses to Epitopes of the Major Surface Glycoprotein ofPneumocystis jiroveciDiffer in Human Immunodeficiency Virus–Infected and Uninfected Persons
| Autor: | Michele S. Hui, Carl J. Fichtenbaum, Peter D. Walzer, Timothy D. Thullen, Robert O’Bert, Michael J. Linke, Reiko Tanaka, Kieran R. Daly, A. G. Smulian |
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| Rok vydání: | 2002 |
| Předmět: |
Opportunistic infection
Blotting Western Molecular Sequence Data HIV Infections Pneumocystis pneumonia Polymerase Chain Reaction Virus Epitope Serology Fungal Proteins Epitopes Escherichia coli medicine Humans Immunology and Allergy Amino Acid Sequence DNA Fungal Antibodies Fungal Membrane Glycoproteins biology Pneumocystis Pneumonia Pneumocystis HIV biology.organism_classification medicine.disease Antigenic Variation Virology Peptide Fragments Recombinant Proteins Infectious Diseases Lentivirus Immunology biology.protein Viral disease Antibody |
| Zdroj: | The Journal of Infectious Diseases. 186:644-651 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/341565 |
| Popis: | The major surface glycoprotein (Msg) of Pneumocystis jiroveci (P. jiroveci) is important in the immunopathogenesis of Pneumocystis pneumonia (PcP), but is difficult to study in humans. We generated 3 overlapping recombinant Msg fragments (MsgA, MsgB and MsgC), and analyzed their reactivity with serum samples from 95 healthy blood donors and 94 human immunodeficiency virus (HIV)-infected persons. Reactivity to the Msg fragments varied with HIV infection and prior episodes of PcP but not with geographic origin. Recognition of MsgA was lower-and recognition of MsgB was significantly lower-in HIV(+) serum compared with donor serum. Serum samples from HIV-positive patients with prior PcP recognized MsgC more frequently than did serum samples from those without PcP. None of the serum samples drawn from 9 patients before they had developed PcP recognized MsgC. These data suggest that these novel recombinant proteins are useful for the analysis of antibody responses to Msg. |
| Databáze: | OpenAIRE |
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