Y-forms as possible intermediates in the replication of measles virus nucleocapsids

Autor:	H. V. Thorne, Evelyn Dermott
Rok vydání:	1977
Předmět:	Messenger RNA Multidisciplinary biology Chemistry viruses Protein subunit RNA Virus Replication biology.organism_classification Virology Measles virus Microscopy Electron Viral Proteins Capsid Transcription (biology) Nucleic acid RNA Viral Protein Binding Single-Stranded RNA
Zdroj:	Nature. 268:345-347
ISSN:	1476-4687 0028-0836
DOI:	10.1038/268345a0
Popis:	THE genome of measles virus, a paramyxovirus, is a non-segmented single stranded RNA molecule which is enclosed within a helix of protein subunits to form a linear nucleocapsid1. Little is known about its mode of replication. It is known, however, for other paramyxoviruses that during the growth cycle transcription of the RNA occurs from an intact nucleocapsid by a virion-associated enzyme2–4. The resulting messenger RNA molecules are less than the full genome length and are unencapsidated5. By contrast, during viral RNA replication no free genome length RNA can be detected5,6 and both plus and minus-stranded RNA are found only in the encapsidated form7. From this evidence, Kingsbury has suggested7 that nucleocapsids of paramyxoviruses replicate without separation of the protein and nucleic acid portions of the template nucleocapsid and has implied that plus and minus-stranded RNAs enclosed within the capsid structure act as templates for each other. If this is so, association of the two types of nucleocapsid in a replicative form should be detectable in infected cells. We have conducted a search by electron microscopy for such structures in measles virus infected cells and the results are described here.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b50642144f7cd8ea9ef68958141b5cc2 https://doi.org/10.1038/268345a0 Zobrazit plný text záznamu