Development of a Subtype-Specific Diagnostic System for Influenza Virus H3N2 Using a Novel Virus-Based Systematic Evolution of Ligands by Exponential Enrichment (Viro-SELEX)
| Autor: | Junyoung Kwon, Han Min Jung, Chandan Narayan, Sung Key Jang, Meehyein Kim, Chonsaeng Kim |
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| Rok vydání: | 2019 |
| Předmět: |
Aptamer
0206 medical engineering Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Computational biology Ligands medicine.disease_cause Influenza Human Influenza A virus medicine Humans General Materials Science Chemistry Oligonucleotide Influenza A Virus H3N2 Subtype SELEX Aptamer Technique Aptamers Nucleotide 021001 nanoscience & nanotechnology 020601 biomedical engineering Novel virus Target protein 0210 nano-technology Molecular probe Systematic evolution of ligands by exponential enrichment |
| Zdroj: | Journal of Biomedical Nanotechnology. 15:1609-1621 |
| ISSN: | 1550-7033 |
| DOI: | 10.1166/jbn.2019.2789 |
| Popis: | Aptamers are oligonucleotide molecules that bind to specific target molecules generated by systematic evolution of ligands by exponential enrichment (SELEX). Aptamers have high future potential for use in diagnostics and therapeutics as molecular probes that recognize target molecules. To develop aptamers against a target protein using a SELEX process, it is necessary to purify the target protein. Purifying a membrane protein, however, is usually a challenging task. Here, we report a novel approach to developing aptamers against membrane proteins. Surrogate viruses containing target proteins on the surface of an enveloped virus (e.g., baculovirus), instead of purified proteins, were used in a new SELEX process. We designated this new SELEX process as "surrogate virus-based SELEX (viro-SELEX)." Using viro-SELEX, we developed a pair of aptamers that specifically interact with the hemagglutinin protein of influenza subtype H3N2. Using the aptamer pair and a lateral flow assay system, we developed a very sensitive point-of-care diagnostic system for specifically detecting influenza virus subtype H3N2. |
| Databáze: | OpenAIRE |
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